School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Hefei 230026, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Sensors (Basel). 2022 Apr 23;22(9):3252. doi: 10.3390/s22093252.
DNA is a type of promising material for the construction of sensors owing to its sequence programmability to control the formation of certain structures. MicroRNA (miRNA) can be applied as promising biomarkers for the diagnosis of a range of diseases. Herein, a novel fluorescent sensing strategy for miRNA is proposed combining duplex-specific nuclease (DSN)-mediated amplification and dumbbell DNA structural switch. Gold nanoparticles (AuNPs) are employed, which provide a 3D reaction interface. They also act as effective fluorescence quenchers. The proposed sensor exhibits high sensitivity (sub-femtomolar level) with a wide dynamic range. In addition, excellent selectivity to distinguish homology sequences is achieved. It also performs satisfactorily in biological samples. Overall, this fluorescent sensor provides a powerful tool for the analysis of miRNA levels and can be applied for related biological studies and clinical diagnosis.
DNA 是一种很有前途的材料，可用于构建传感器，因为其序列可编程性可控制某些结构的形成。microRNA（miRNA）可用作一系列疾病诊断的有前途的生物标志物。在此，提出了一种新的荧光传感策略，将双链特异性核酸酶（DSN）介导的扩增与哑铃 DNA 结构开关相结合。使用金纳米粒子（AuNPs）作为 3D 反应界面，它们还可以作为有效的荧光猝灭剂。所提出的传感器具有高灵敏度（亚飞摩尔级）和宽动态范围。此外，还实现了区分同源序列的优异选择性。它在生物样品中也表现良好。总的来说，这种荧光传感器为 miRNA 水平的分析提供了一种强大的工具，可用于相关的生物学研究和临床诊断。
