Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
Jinan Guoke Medical Technology Development Co., Ltd., Jinan 250103, China.
Anal Chem. 2023 Mar 7;95(9):4564-4569. doi: 10.1021/acs.analchem.3c00087. Epub 2023 Feb 22.
Highly sensitive and selective detection of microRNA (miRNA) is becoming more and more important in the discovery, diagnosis, and prognosis of various diseases. Herein, we develop a three-dimensional DNA nanostructure based electrochemical platform for duplicate detection of miRNA amplified by nicking endonuclease. Target miRNA first helps construction of three-way junction structures on the surfaces of gold nanoparticles. After nicking endonuclease-powered cleavage reactions, single-stranded DNAs labeled with electrochemical species are released. These strands can be facilely immobilized at four edges of the irregular triangular prism DNA (iTPDNA) nanostructure via triplex assembly. By evaluating the electrochemical response, target miRNA levels can be determined. In addition, the triplexes can be disassociated by simply changing pH conditions, and the iTPDNA biointerface can be regenerated for duplicate analyses. The developed electrochemical method not only exhibits an excellent prospect in the detection of miRNA but also may inspire the engineering of recyclable biointerfaces for biosensing platforms.
高灵敏度和选择性检测 microRNA（miRNA）在各种疾病的发现、诊断和预后中变得越来越重要。在此，我们开发了一种基于三维 DNA 纳米结构的电化学生物传感平台，用于通过切口酶扩增的 miRNA 的双重检测。靶 miRNA 首先帮助在金纳米粒子表面构建三链结结构。在切口酶驱动的切割反应后，释放出标记有电活性物质的单链 DNA。这些链可以通过三聚体组装轻易地固定在不规则三角棱柱 DNA（iTPDNA）纳米结构的四个边缘上。通过评估电化学响应，可以确定靶 miRNA 的水平。此外，只需改变 pH 值条件，三聚体就可以解离，并且可以再生 iTPDNA 生物界面以进行重复分析。所开发的电化学生物传感方法不仅在 miRNA 的检测中具有优异的前景，而且可能启发用于生物传感平台的可回收生物界面的工程设计。
