Fite Tarekegn, Tefera Tadele, Goftishu Muluken, Damte Tebekew
International Centre of Insect Physiology and Ecology (ICIPE) Addis Ababa Ethiopia.
School of Plant Sciences College of Agriculture and Environmental Sciences Haramaya University Dire Dhawa Ethiopia.
Ecol Evol. 2022 May 13;12(5):e8907. doi: 10.1002/ece3.8907. eCollection 2022 May.
The Old World bollworm, (Hubner) (Lepidoptera: Noctuidae), is a globally distributed agricultural and horticultural insect pest. Despite the economic importance of this insect in Ethiopia, its genetic diversity and demographic history are poorly understood. We examined the nucleotide variation of the mitochondrial cytochrome c oxidase subunit I (COI) gene fragment of 74 . individuals from six collection sites in Ethiopia. We recorded 15 COI haplotypes in . , ten globally shared and five exclusive to Ethiopia (HaET15, HaET14, HaET10, HaET7, and HaET4). Haplotype HaET1 was the most widely geographically distributed and frequent (71.62%). Analysis of molecular variance (AMOVA) revealed a high and significant level of variation within . populations ( = -0.0135). Negative values of the neutrality test and nonsignificant index of mismatch distribution supported the demographic expansion of H. armigera populations in Ethiopia; furthermore, this was also supported by the nonsignificant values of the sum of squared deviations (SSD) and raggedness index (r). The high genetic variation and population expansion of . have immense implications for devising locally adapted management strategies in area-wide integrated pest management IPM programs. However, a comprehensive study of . genetic diversity and population structure using various molecular markers is needed for future confirmation.
棉铃虫（Helicoverpa armigera (Hubner)，鳞翅目：夜蛾科）是一种分布于全球的农业和园艺害虫。尽管这种昆虫在埃塞俄比亚具有重要的经济意义，但其遗传多样性和种群历史却鲜为人知。我们检测了来自埃塞俄比亚六个采集地点的74只棉铃虫个体线粒体细胞色素c氧化酶亚基I（COI）基因片段的核苷酸变异。我们在棉铃虫中记录了15种COI单倍型，其中10种是全球共享的，5种是埃塞俄比亚特有的（HaET15、HaET14、HaET10、HaET7和HaET4）。单倍型HaET1在地理分布上最广且最为常见（71.62%）。分子方差分析（AMOVA）显示棉铃虫种群内部存在高度且显著的变异水平（FST = -0.0135）。中性检验的负值和不显著的错配分布指数支持了埃塞俄比亚棉铃虫种群的扩张；此外，平方偏差总和（SSD）和参差不齐指数（r）的不显著值也支持了这一点。棉铃虫的高遗传变异和种群扩张对于在区域综合虫害管理（IPM）计划中制定因地制宜的管理策略具有重大意义。然而，未来还需要使用各种分子标记对棉铃虫的遗传多样性和种群结构进行全面研究以作进一步确认。
