Seow W K, Smith S E, McCormack J G, Thong Y H
J Immunol Methods. 1987 Apr 2;98(1):113-8. doi: 10.1016/0022-1759(87)90443-1.
The accumulation of 2-deoxy-D-[1-3H]glucose, a non-metabolised analogue of glucose provides a quantitative measurement of the state of activation of phagocytic cells. A microassay for 3H-deoxyglucose uptake by human neutrophils and monocytes is described. Optimal conditions for the assay include the use of 5 X 10(5) cells and 0.78 microCi/ml of deoxyglucose in a final volume of 0.2 ml per microtitre well, and an incubation time of 30 min at 37 degrees C. This simple, rapid and reproducible technique may find application in experimental immunology.
2-脱氧-D-[1-³H]葡萄糖（一种葡萄糖的非代谢类似物）的积累提供了吞噬细胞激活状态的定量测量方法。本文描述了一种用于检测人中性粒细胞和单核细胞摄取³H-脱氧葡萄糖的微量测定法。该测定法的最佳条件包括在每微孔板孔最终体积为0.2 ml中使用5×10⁵个细胞和0.78微居里/毫升的脱氧葡萄糖，以及在37℃下孵育30分钟。这种简单、快速且可重复的技术可能在实验免疫学中得到应用。
