Basavarajappa Lokesh, Hoyt Kenneth
Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA.
Photoacoustics. 2022 May 9;26:100365. doi: 10.1016/j.pacs.2022.100365. eCollection 2022 Jun.
A photoacoustic (PA) graphic equalization (PAGE) algorithm was developed to characterize the relative size of optical absorbing aggregates. This technique divides the PA signal into frequency bands related to different-sized optical absorbers. Simulations of a material containing optical absorbing microparticles of varying size were used to assess PAGE performance. Experiments were performed on phantom materials containing microspheres of varying size and concentration. Additional experiments were performed using tubes with fresh clotting blood. PA data was obtained using a Vevo LAZR-X system (FUJIFILM VisualSonics Inc). PAGE imaging of phantoms with varying-sized optical absorbers found a 1.5-fold difference in mean image intensity ( < 0.001). Conversely, PA images from these same materials exhibited no intensity changes ( = 0.68). PAGE imaging results from clotting blood exhibited differences for clot sizes in the range 0.30-0.64 mm ( < 0.001). In summary, PAGE imaging can distinguish optical absorbing aggregates of varying size.
开发了一种光声（PA）图形均衡（PAGE）算法来表征光吸收聚集体的相对大小。该技术将PA信号划分为与不同大小光吸收体相关的频带。使用包含不同大小光吸收微粒的材料模拟来评估PAGE性能。对含有不同大小和浓度微球的体模材料进行了实验。使用装有新鲜凝血的管子进行了额外实验。使用Vevo LAZR-X系统（富士胶片视觉超声公司）获取PA数据。对具有不同大小光吸收体的体模进行PAGE成像，发现平均图像强度存在1.5倍差异（<0.001）。相反，来自这些相同材料的PA图像未显示强度变化（=0.68）。凝血的PAGE成像结果显示，凝块大小在0.30 - 0.64毫米范围内存在差异（<0.001）。总之，PAGE成像可以区分不同大小的光吸收聚集体。
