Department of Neurology, Wenzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Wenzhou, China.
The Third School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, China.
J Ethnopharmacol. 2022 Sep 15;295:115379. doi: 10.1016/j.jep.2022.115379. Epub 2022 May 18.
Xiao-Xu-Ming Decoction (XXMD) is a classical Chinese medicinal compound for the treatment of ischemic stroke, which has good efficacy in clinical studies and also plays a neuroprotective role in pharmacological studies.
The purpose of this study is to investigate the potential and integral interventional effects of XXMD on cerebral ischemia/reperfusion rat model.
In this study, H NMR metabolomics was used, combined with neurological functional assessments, cerebral infarct area measurements, and pathological staining including Nissl staining, immunofluorescence staining of NeuN and TUNEL, and immunohistochemical staining of MCT2, to analyze the metabolic effects of XXMD in the treatment of an ischemia/reperfusion rat model.
It's observed that XXMD treatment could improve the neurological deficit scores and reduce the cerebral infarct areas on cerebral ischemia/reperfusion rat model. The pathological staining results performed that XXMD treatment could improve the decrease of Nissl bodies and the expression of NeuN and MCT2, reduce the high expression of TUNEL. In H NMR study, it revealed that the metabolic patterns among three experimental groups were different, the level of lactate, acetate, NAA, glutamate, and GABA were improved to varying degrees in different brain area.
Our findings indicated that XXMD has positive effect on neuroprotection and improvement of metabolism targeting cerebral ischemic injury in rats, which showed great potential for ischemic stroke.
消眩止晕汤（XXMD）是一种治疗缺血性脑卒中的经典中药复方，在临床研究中疗效显著，在药理学研究中也具有神经保护作用。
本研究旨在探讨 XXMD 对脑缺血/再灌注大鼠模型的潜在综合干预作用。
本研究采用 H NMR 代谢组学方法，结合神经功能评估、脑梗死面积测量以及尼氏染色、NeuN 和 TUNEL 免疫荧光染色、MCT2 免疫组织化学染色等病理染色，分析 XXMD 治疗缺血/再灌注大鼠模型的代谢效应。
XXMD 治疗可改善脑缺血/再灌注大鼠的神经功能缺损评分，减少脑梗死面积。病理染色结果表明，XXMD 治疗可改善尼氏小体减少和 NeuN 和 MCT2 表达降低，减少 TUNEL 高表达。在 H NMR 研究中，揭示了三个实验组之间的代谢模式存在差异，XXMD 治疗可在不同脑区不同程度地改善乳酸盐、醋酸盐、NAA、谷氨酸和 GABA 水平。
本研究结果表明，XXMD 对大鼠缺血性脑损伤具有神经保护和改善代谢的积极作用，为缺血性脑卒中的治疗提供了广阔的应用前景。
