金-硫键纳米探针用于血清中前列腺特异性抗原的检测。
Au-Se bonded nanoprobe for prostate specific antigen detection in serum.
机构信息
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, 250014, PR China.
Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, PR China.
出版信息
Anal Chim Acta. 2022 Jun 1;1210:339852. doi: 10.1016/j.aca.2022.339852. Epub 2022 Apr 25.
The detection of prostate specific antigen (PSA) in serum can realize early diagnosis of prostate cancer and prevent the occurrence of prostate tumors, as well as offering guidance during the therapy. Herein, a Au-Se bonded nanoprobes that can specifically detect PSA was designed and constructed. The peptide chains that can be specifically cleaved by PSA were firstly functionalized with fluorescent dye and selenol, and then bind to the Au nanoparticles to produce the probe. The dye's fluorescence was quenched due to the FRET effect, but recovered by PSA's cutting. The nanoprobe can detect PSA in serum with extraordinary anti-interference ability against other proteins (detection range 1-40 ng/mL). This work provides a new method for the detection of PSA in serum, and has potential guiding significance for clinical PSA detection.
血清前列腺特异抗原（PSA）的检测可实现前列腺癌的早期诊断和预防前列腺肿瘤的发生，并为治疗提供指导。为此，设计并构建了一种可以特异性检测 PSA 的 Au-Se 键合纳米探针。首先，将可被 PSA 特异性切割的肽链用荧光染料和硒醇官能化，然后结合到 Au 纳米颗粒上生成探针。由于 FRET 效应，染料的荧光被猝灭，但被 PSA 的切割所恢复。该纳米探针可在血清中检测 PSA，对其他蛋白质具有出色的抗干扰能力（检测范围为 1-40ng/mL）。这项工作为血清中 PSA 的检测提供了一种新方法，对临床 PSA 检测具有潜在的指导意义。
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