Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Unidad de Biotecnología Vegetal, 44270, Guadalajara, Mexico.
Graduate Program in Biosciences, Life Science Division, University of Guanajuato Campus Irapuato-Salamanca, 36500, Irapuato, Guanajuato, Mexico.
Arch Microbiol. 2022 May 20;204(6):344. doi: 10.1007/s00203-022-02938-2.
Thurincin H is a bacteriocin produced by Bacillus thuringiensis, it is encoded in a group of ten genes, most of which have been characterized experimentally or by homology. However, the activity of the thnI gene encoding a 95 amino acid ORF remains unknown. In this work, the thnI gene was cloned under the regulation of two promoters and transformed into a sensitive strain to determine if it acts as an immunity protein. In addition, a deletion mutant without the thnI gene was used to test whether thnI is required or not for the biosynthesis of thurincin H. It was concluded that thnI does not provide immunity and is not required to produce thurincin H.
苏云金菌素 H 是由苏云金芽孢杆菌产生的细菌素，它由十个基因编码，其中大多数已通过实验或同源性进行了表征。然而，编码 95 个氨基酸 ORF 的 thnI 基因的活性仍然未知。在这项工作中，thnI 基因在两个启动子的调控下被克隆，并转化到敏感菌株中，以确定它是否作为一种免疫蛋白发挥作用。此外，还使用没有 thnI 基因的缺失突变体来测试 thnI 是否是苏云金菌素 H 生物合成所必需的。结论是 thnI 不提供免疫，并且不要求产生苏云金菌素 H。
