利用劳丹通用偏振测量法研究渗透胁迫对活细胞膜的影响。
Effect of osmotic stress on live cell plasma membranes, probed via Laurdan general polarization measurements.
机构信息
Department of Materials Science and Engineering, Institute for NanoBioTechnology, and Program in Molecular Biophysics, Johns Hopkins University, Baltimore, Maryland.
Department of Materials Science and Engineering, Institute for NanoBioTechnology, and Program in Molecular Biophysics, Johns Hopkins University, Baltimore, Maryland.
出版信息
Biophys J. 2022 Jun 21;121(12):2411-2418. doi: 10.1016/j.bpj.2022.05.016. Epub 2022 May 19.
Here we seek to gain insight into changes in the plasma membrane of live cells upon the application of osmotic stress using Laurdan, a fluorescent probe that reports on membrane organization, hydration, and dynamics. It is known that the application of osmotic stress to lipid vesicles causes a decrease in Laurdan's generalized polarization (GP), which has been interpreted as an indication of membrane stretching. In cells, we see the opposite effects, as GP increases when the osmolarity of the solution is decreased. This increase in GP is associated with the presence of caveolae, which are known to disassemble and flatten in response to osmotic stress.
在这里，我们使用劳丹胺（一种报告膜组织、水合和动态的荧光探针）研究了在施加渗透胁迫时活细胞的质膜变化。已知向脂质体施加渗透胁迫会导致劳丹胺的广义极化（GP）降低，这被解释为膜拉伸的指示。在细胞中，我们看到相反的效果，当溶液渗透压降低时，GP 增加。这种 GP 的增加与 caveolae 的存在有关，已知 caveolae 会在渗透胁迫下解体和平坦化。
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