Hou Zongkun, Meng Run, Chen Ganghua, Lai Tangmin, Qing Rui, Hao Shilei, Deng Jia, Wang Bochu
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400030, China.
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Sci Total Environ. 2022 Sep 10;838(Pt 2):155811. doi: 10.1016/j.scitotenv.2022.155811. Epub 2022 May 18.
Plastic particles, especially nanoplastics, represent an emerging concern of threat to human health, oral uptake is an important pathway for the plastic particles ingestion by human. While their fate and adverse effects in animal gastrointestinal tract are increasingly investigated, knowledge about their uptake and toxicity in human intestine is still limited. Here, by exposing human intestinal organoids to polystyrene nanoplastics (PS-NPs, ~50 nm in size) with concentrations of 10 and 100 μg/mL, we present evidence of their distinct accumulation in various type cells in intestinal organoids, then causing the cell apoptosis and inflammatory response. Our results further revealed that the effective inhibition of PS-NPs accumulation in secretive cells through co-exposure to a clathrin-mediated endocytosis inhibitor (chlorpromazine), and proved the essential role of active endocytosis in the PS-NPs uptaking into enterocyte cells. Our work not only elucidated the potential uptake and toxicity of PS-NPs in human intestinal cells and the underlying mechanism, but also provide a potential therapeutic approach to relieve the toxicity of PS-NPs to human through the endocytosis inhibition.
塑料颗粒，尤其是纳米塑料，是对人类健康构成威胁的一个新问题，经口摄入是人类摄入塑料颗粒的重要途径。虽然它们在动物胃肠道中的归宿和不良影响受到越来越多的研究，但关于它们在人体肠道中的摄取和毒性的知识仍然有限。在这里，通过将人肠道类器官暴露于浓度为10和100μg/mL的聚苯乙烯纳米塑料（PS-NPs，尺寸约50nm）中，我们展示了它们在肠道类器官的各种类型细胞中明显积累的证据，进而导致细胞凋亡和炎症反应。我们的结果进一步表明，通过共同暴露于网格蛋白介导的内吞作用抑制剂（氯丙嗪）可有效抑制PS-NPs在分泌细胞中的积累，并证明了主动内吞作用在PS-NPs进入肠细胞中的重要作用。我们的工作不仅阐明了PS-NPs在人体肠道细胞中的潜在摄取和毒性以及潜在机制，还提供了一种通过抑制内吞作用来减轻PS-NPs对人体毒性的潜在治疗方法。
