Department of Organic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University (TMDU), 2-3-10 Kanda-Surugadai, Chiyoda, Tokyo, 101-0062, Japan.
Department of Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo, Tokyo, 113-8549, Japan.
Macromol Biosci. 2022 Aug;22(8):e2200115. doi: 10.1002/mabi.202200115. Epub 2022 May 31.
Polyetheretherketone (PEEK) is a candidate material for bone implants as an alternative to metals. However, PEEK exhibits poor osseointegration and low endothelial compatibility. This study demonstrates the phototethering of collagen onto PEEK surfaces to facilitate osteoblastic and vascular endothelial performance. In particular, collagen with methacryloyl groups is covalently tethered to the PEEK surface via surface-initiated photopolymerization. This process is simpler than the conventional method of collagen-tethering and can be extended to the surface-patterning treatment of collagen. The collagen is confirmed to be tethered to the PEEK surface using attenuated total reflection Fourier transform infrared measurements, bicinchoninic acid assays, and atomic force microscopic observations. When human bone marrow-derived mesenchymal stem cells (HbmMSCs) are cultured on collagen-tethered PEEK (COL-PEEK) surfaces, the cells favorably adhere and proliferate. After inducing osteogenic differentiation, the cells on the COL-PEEK surfaces show higher expression levels of osteoblast-related genes and mineralization than those on the PEEK surface. Moreover, the tethering of collagen greatly improves endothelial proliferation. The COL-PEEK surfaces promotes endothelial networking in coculture with HbmMSCs. These results suggest that COL-PEEK is highly compatible with both osteoblasts and vascular endothelial cells. COL-PEEK is a promising implant that induces osteogenesis and angiogenesis to repair bone tissues.
聚醚醚酮（PEEK）是一种替代金属的骨植入物候选材料。然而，PEEK 表现出较差的骨整合和低内皮相容性。本研究展示了将胶原蛋白光固定到 PEEK 表面以促进成骨细胞和血管内皮细胞性能的方法。具体而言，通过表面引发光聚合将具有甲基丙烯酰基的胶原蛋白共价固定到 PEEK 表面。该过程比传统的胶原蛋白固定方法更简单，并且可以扩展到胶原蛋白的表面图案处理。使用衰减全反射傅里叶变换红外测量、双缩脲分析和原子力显微镜观察证实了胶原蛋白被固定到 PEEK 表面上。当人骨髓间充质干细胞（HbmMSCs）在胶原蛋白固定的 PEEK（COL-PEEK）表面上培养时，细胞有利地附着和增殖。在诱导成骨分化后，COL-PEEK 表面上的细胞表现出比 PEEK 表面更高的成骨相关基因表达水平和矿化。此外，胶原蛋白的固定大大提高了内皮细胞的增殖。COL-PEEK 表面促进与 HbmMSCs 共培养中的内皮网络形成。这些结果表明 COL-PEEK 与成骨细胞和血管内皮细胞高度兼容。COL-PEEK 是一种有前途的植入物，可诱导成骨和血管生成以修复骨组织。
