结直肠癌中FoxP3调节性T细胞、CD66b肿瘤相关中性粒细胞和CD163肿瘤相关巨噬细胞浸润之间的相关性
The correlation between infiltration of FoxP3 Tregs, CD66b TANs and CD163 TAMs in colorectal cancer.
作者信息
Ren Yuehan, Chen Zhiyuan, Sun Jiancheng, Cai Yiqi, Chen Zhejing, Chen Xiaolei, Wu Wenyi
机构信息
Department of Colorectal and Anal Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
Department of Microbiology and Immunology, School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
出版信息
Cent Eur J Immunol. 2022;47(1):1-7. doi: 10.5114/ceji.2022.114004. Epub 2022 Mar 10.
INTRODUCTION
The infiltration of immune cells in tumor tissue is affected by the tumor microenvironment. However, the relationship between the infiltration of regulatory T cells (Tregs), tumor-associated neutrophils (TANs) and tumor-associated macrophages (TAMs) in colorectal cancer (CRC) remains unclear.
MATERIAL AND METHODS
Tissue microarray and immunohistochemistry were used to detect the infiltration of FoxP3 Tregs, CD66b TANs and CD163 TAMs in 249 CRC samples (training cohort) and 243 CRC samples (validation cohort). The relationship between two cells was evaluated by Spearman's rank correlation coefficient and comparison between two groups was analyzed by Mann-Whitney U test.
RESULTS
The continuous variable positive cell numbers were non-normally distributed. Spearman correlation analysis showed that CD66b TAN level in cancer tissues was negatively related to FoxP3 Treg level (correlation coefficient: -0.495, p < 0.05) and CD163 TAM level (correlation coefficient: -0.266, p < 0.05), and FoxP3 Treg level was positively related to CD163 TAM level (correlation coefficient: 0.467, p < 0.05) in the training cohort. The numbers of FoxP3 Tregs were significantly different between low and high CD66b TAN level groups (p < 0.001), as well as that of CD66b TANs in low and high CD163 TAM level groups and CD163 TAMs in different FoxP3 Treg level groups. The results of the validation cohort were similar to those of the training cohort.
CONCLUSIONS
There is a negative correlation between infiltration of CD66b TANs and that of FoxP3 Tregs or CD163 TAMs, and a positive correlation between infiltration of FoxP3 Tregs and CD163 TAMs in CRC tissues.
引言
肿瘤组织中免疫细胞的浸润受肿瘤微环境影响。然而，结直肠癌（CRC）中调节性T细胞（Tregs）、肿瘤相关中性粒细胞（TANs）和肿瘤相关巨噬细胞（TAMs）浸润之间的关系仍不清楚。
材料与方法
采用组织芯片和免疫组化法检测249例CRC样本（训练队列）和243例CRC样本（验证队列）中FoxP3 Tregs、CD66b TANs和CD163 TAMs的浸润情况。通过Spearman等级相关系数评估两种细胞之间的关系，采用Mann-Whitney U检验分析两组之间的差异。
结果
连续变量阳性细胞数呈非正态分布。Spearman相关性分析显示，训练队列中癌组织CD66b TAN水平与FoxP3 Treg水平呈负相关（相关系数：-0.495，p<0.05），与CD163 TAM水平呈负相关（相关系数：-0.266，p<0.05），而FoxP3 Treg水平与CD163 TAM水平呈正相关（相关系数：0.467，p<0.05）。低CD66b TAN水平组与高CD66b TAN水平组之间FoxP3 Tregs数量差异有统计学意义（p<0.001），低CD163 TAM水平组与高CD163 TAM水平组之间CD66b TANs数量以及不同FoxP3 Treg水平组之间CD163 TAMs数量差异均有统计学意义。验证队列结果与训练队列相似。
结论
CRC组织中CD66b TANs浸润与FoxP3 Tregs或CD163 TAMs浸润呈负相关，FoxP3 Tregs浸润与CD163 TAMs浸润呈正相关。
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