George Sherly Deborah, Archana Rajagopalan, Parasuraman Subramani
Department of Physiology, Faculty of Medicine, Manipal University College Malaysia, Melaka, Malaysia.
Department of Physiology, Saveetha Medical College, Saveetha Institute of Technical and Medical Sciences (SIMATS), Chennai, India.
Front Pharmacol. 2022 Apr 27;13:834292. doi: 10.3389/fphar.2022.834292. eCollection 2022.
Caloric Vestibular Stimulation (CVS) is a non-invasive technique for stimulating the vestibular system. The vestibular system maintains equilibrium and acts as a moderator of mood, emotional control, and stress levels. Stress is a disruption of psychological, behavioral, and physiological homeostasis that affects people of all ages in today's world. Thus, modest therapeutic procedures like vestibular stimulation can be practiced to effectively reduce stress. Hence, the purpose of the study was to determine the effect of vestibular stimulation on improving behavioral alterations and neurotrophic factors in rats exposed to . The study employed 24 healthy male Sprague Dawley rats divided into four groups ( = 6). CMS was induced for 28 days with a variety of stimuli. Bilateral CVS with hot water (temperature ≈40°C) was started on Day 14 of CMS and continued for 15 days. On days 1, 15, and 28, locomotor activity (LA), wire grip strength (WGS), fall off time (FT), and immobilization time (IT) were measured, and the data were analyzed statistically. Additionally, neurotrophic factors such as Brain Derived Neurotrophic Factor (BDNF) and Glial cell line-Derived Neurotrophic Factor (GDNF) were observed in rats' hippocampus. On days 15 and 28, the CMS-induced group showed a significant reduction in LA, WGS, FT and IT in comparison to the control group. On day 28, the CVS-induced group demonstrated a significant increase in WGS, FT and IT when compared to the CMS group. Immunohistochemical analysis revealed that animals subjected to CMS had decreased BDNF and GDNF expression compared to the control group, indicating neuronal dysfunction in the hippocampus in response to stress. However, therapy with CVS increased BDNF and GDNF expression, thereby regenerating damaged hippocampus nerve terminals. The findings of the current study revealed that CVS is a safe and simple neuroprotective treatment against stress and a promising non-invasive technique for overcoming the motor symptoms associated with it. The findings may pave the way for future research and therapeutic applications of CVS for stress management.
热前庭刺激（CVS）是一种刺激前庭系统的非侵入性技术。前庭系统维持平衡，并对情绪、情绪控制和压力水平起到调节作用。压力是心理、行为和生理稳态的破坏，在当今世界影响着各个年龄段的人。因此，可以采用如前庭刺激这样适度的治疗程序来有效减轻压力。因此，本研究的目的是确定前庭刺激对改善暴露于……的大鼠行为改变和神经营养因子的影响。该研究使用了24只健康的雄性Sprague Dawley大鼠，分为四组（每组 = 6只）。通过多种刺激诱导慢性应激模型（CMS）28天。在CMS第14天开始用热水（温度约40°C）进行双侧CVS，并持续15天。在第1天、第15天和第28天，测量运动活动（LA）、握力（WGS）、跌落时间（FT）和固定时间（IT），并对数据进行统计学分析。此外，在大鼠海马中观察神经营养因子，如脑源性神经营养因子（BDNF）和胶质细胞系源性神经营养因子（GDNF）。在第15天和第28天，与对照组相比，CMS诱导组的LA、WGS、FT和IT显著降低。在第28天，与CMS组相比，CVS诱导组的WGS、FT和IT显著增加。免疫组织化学分析显示，与对照组相比，遭受CMS的动物BDNF和GDNF表达降低，表明海马体中神经元功能因应激而受损。然而，CVS治疗增加了BDNF和GDNF表达，从而使受损的海马神经末梢再生。本研究结果表明，CVS是一种安全、简单的抗应激神经保护治疗方法，是一种有前景的非侵入性技术，可克服与之相关的运动症状。这些发现可能为CVS在压力管理方面的未来研究和治疗应用铺平道路。
