Zhang Xianlin, Huang Juan, Li Jinlong, Lu Qiao, Huang Yuli, Lu Dongyu, Tang Yang, Zhu Jian, Zhuang Jianhui
Department of Cardiology, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, People's Republic of China.
Health Management Centre, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, People's Republic of China.
Int J Gen Med. 2022 May 16;15:4975-4983. doi: 10.2147/IJGM.S366956. eCollection 2022.
Atrial fibrillation (AF) is the most common sustained arrhythmia with a high rate of recurrence after catheter ablation. The gene encoding transcription factor 21 () has been linked to coronary artery disease risk by human genome-wide association studies in multiple racial ethnic groups. However, the association of with AF remains unclear.
Circulating leukocytes in patients with paroxysmal AF (PAF) and 92 age-matched controls without a history of cardiovascular disease, AF and other arrhythmias were collected. A total of 224 PAF patients receiving radiofrequency ablation had an 18-month scheduled follow-up study for recurrence of AF. Three single-nucleotide polymorphisms (SNPs) of ( and ) were genotyped by PCR, and serum levels of were measured by ELISA.
More males and smokers were observed in the PAF group compared with controls. C allele of , G allele and GG genotype of were markedly associated with the increased onset of PAF. The levels of serum TCF21 were significantly higher in PAF group than those in control group (1.96 ± 0.85 vs 0.86 ± 0.49 ng/mL, <0.001). Based on logistic regression analysis, we confirmed that risk allele at and serum TCF21 concentration were independently correlated with the incidence of PAF. Furthermore, GG genotype of enhanced the susceptibility of AF recurrence after ablation.
G allele and GG genotype of in and elevated TCF21 concentration are significantly associated with the onset of PAF and recurrence after ablation.
心房颤动（AF）是最常见的持续性心律失常，导管消融术后复发率很高。通过对多个种族群体进行的全基因组关联研究，编码转录因子21（TCF21）的基因已与冠状动脉疾病风险相关联。然而，TCF21与AF之间的关联仍不明确。
收集阵发性AF（PAF）患者及92名年龄匹配、无心血管疾病、AF及其他心律失常病史的对照者的循环白细胞。共有224例接受射频消融的PAF患者进行了为期18个月的AF复发随访研究。通过聚合酶链反应（PCR）对TCF21的三个单核苷酸多态性（SNP）（rs12190287和rs7903146）进行基因分型，并采用酶联免疫吸附测定（ELISA）法检测血清TCF21水平。
与对照组相比，PAF组男性和吸烟者更多。rs12190287的C等位基因、rs7903146的G等位基因和GG基因型与PAF发病增加显著相关。PAF组血清TCF21水平显著高于对照组（1.96±0.85 vs 0.86±0.49 ng/mL，P<0.001）。基于逻辑回归分析，我们证实rs12190287的风险等位基因和血清TCF21浓度与PAF发病率独立相关。此外，rs7903146的GG基因型增加了消融术后AF复发的易感性。
TCF21基因中rs7903146的G等位基因和GG基因型以及TCF21浓度升高与PAF发病及消融术后复发显著相关。
