Enhanced Catalytic Activity of a New Nanobiocatalytic System Formed by the Adsorption of Cytochrome on Pluronic Triblock Copolymer Stabilized MoS Nanosheets.

The formation of nanobiohybrids through the immobilization of enzymes on functional nanomaterials has opened up exciting research opportunities at the nanobiointerfaces. These systems hold great promise for a wide range of applications in biosensing, biocatalytic, and biomedical fields. Here, we report the formation of a hybrid nanobiocatalytic system through the adsorption of cytochrome (Cyt ) on pluronic triblock copolymer, P123 (PEO--PPO--PEO), stabilized MoS nanosheets. The use of pluronic polymer has helped not only to greatly stabilize the exfoliated MoS nanosheets but also to allow easy adsorption of Cyt on the nanosheets without major structural changes due to its excellent biocompatibility and soft protein-binding property. By comparing the catalytic activity of the Cyt -MoS nanobiohybrid with that of the free Cyt and as-prepared MoS nanosheets, we have demonstrated the active role of the nanobiointeractions in enhancing the catalytic activity of the hybrid. Slight structural perturbation at the active site of the Cyt upon adsorption on MoS has primarily facilitated the peroxidase activity of the Cyt . As the MoS nanosheets and the native Cyt individually exhibit weaker intrinsic peroxidase activities, their mutual modulation at the nanobiointerface has made the Cyt -MoS a novel nanobiocatalyst with superior activity.