Reid D M, Forrester J V, Campbell A M
J Clin Lab Immunol. 1986 Dec;21(4):165-8.
The Royal College of Surgeons (RCS) rat carries a recessive gene for retinal dystrophy and is used as an experimental model for human retinal degenerative conditions such as retinitis pigmentosa (RP). It has been suggested that an autoimmune response to retinal antigens may contribute to the retinal degenerative processes. In this study, fusions of RCS rat splenic lymphocytes with the rat Y3 Ag 1.2.3 myeloma cell line, resulted in primary fusion cultures, some of which were positive to retinal S-antigen and others to insoluble components of rod outer segments. Three fully cloned IgM monoclonal antibodies were generated and the reaction of 2 of them to S-antigen and opsin was confirmed by immunoblotting.
皇家外科学院（RCS）大鼠携带有视网膜营养不良的隐性基因，被用作人类视网膜退行性疾病如色素性视网膜炎（RP）的实验模型。有人提出，对视网膜抗原的自身免疫反应可能导致视网膜退行性病变过程。在本研究中，RCS大鼠脾淋巴细胞与大鼠Y3 Ag 1.2.3骨髓瘤细胞系融合，产生了原代融合培养物，其中一些对视网膜S抗原呈阳性，另一些对视杆外段的不溶性成分呈阳性。产生了三种完全克隆的IgM单克隆抗体，其中两种与S抗原和视蛋白的反应通过免疫印迹得到证实。
