Hirakawa Hidetada, Suzue Kazutomo, Uchida Motoyuki, Takita Ayako, Kamitani Wataru, Tomita Haruyoshi
Department of Bacteriology, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Department of Infectious Diseases and Host Defense, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Front Microbiol. 2022 May 6;13:883689. doi: 10.3389/fmicb.2022.883689. eCollection 2022.
Enterohemorrhagic (EHEC) is one of the most common foodborne pathogens. However, no drug that prevents the severe complications caused by this bacterium has been approved yet. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC) adsorbs Shiga toxins, and Type III secretory EspA/EspB proteins responsible for EHEC pathogenesis, and decreases the extracellular levels of these proteins. On the other hand, this material did not affect the growth of EHEC. traditionally used to estimate Type III secretion system-associated virulence in mice is highly virulent. The survival period of infected mice was prolonged when MgOC was administered. This adsorbent disturbed neither mammalian cells nor normal intestinal bacteria, such as , , and . In contrast, MgOC adsorbed antimicrobial agents, including β-lactams, quinolones, tetracyclines, and trimethoprim/sulfamethoxazole. However, fosfomycin and amikacin were not adsorbed. Thus, MgOC can be used with fosfomycin and amikacin to treat infections. MgOC is used for industrial purposes, such as an electrode catalyst, a bioelectrode, and enzyme immobilization. The study proposed another potential application of MgOC, assisting anti-EHEC chemotherapy.
肠出血性大肠杆菌（EHEC）是最常见的食源性病原体之一。然而，目前尚未有获批的药物能够预防这种细菌引起的严重并发症。本研究表明，一种大孔氧化镁（MgO）模板碳材料（MgOC）能够吸附志贺毒素以及负责EHEC致病机制的III型分泌EspA/EspB蛋白，并降低这些蛋白的细胞外水平。另一方面，这种材料并不影响EHEC的生长。传统上用于评估小鼠中III型分泌系统相关毒力的方法具有高毒性。当给予MgOC时，感染小鼠的存活期得以延长。这种吸附剂既不干扰哺乳动物细胞，也不干扰诸如[此处原文缺失具体细菌名称]等正常肠道细菌。相比之下，MgOC会吸附包括β-内酰胺类、喹诺酮类、四环素类以及甲氧苄啶/磺胺甲恶唑在内的抗菌剂。然而，磷霉素和阿米卡星不会被吸附。因此，MgOC可与磷霉素和阿米卡星联合使用来治疗感染。MgOC用于工业用途，例如作为电极催化剂、生物电极以及酶固定化。该研究提出了MgOC的另一个潜在应用，即辅助抗EHEC化疗。
