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麦醇溶蛋白与茶多酚相互作用:潜在应用与作用机制。

Gliadin interacted with tea polyphenols: potential application and action mechanism.

机构信息

Antioxidant Polyphenols Team, Department of Food Engineering, Sichuan University, Chengdu, PR China.

The Key Laboratory of Food Science and Technology of Sichuan Province of Education, Sichuan University, Chengdu, PR China.

出版信息

Int J Food Sci Nutr. 2022 Sep;73(6):786-799. doi: 10.1080/09637486.2022.2078283. Epub 2022 May 23.

Abstract

The effect of tea polyphenols (TPs) on noodles quality was investigated, and the interaction mechanism between catechins and gliadins was explored. With TPs addition, noodles showed the significant changes in physicochemical and sensory properties. The water absorption, tensile strength and elasticity increased by 1.35%, 4.98%, 28.51% with 0.5% of TPs, and then decreased with the increasing of TPs. According to the determinations of surface hydrophobicity, spatial structure, thermal properties, amidogen and sulfhydryl content, the structure and properties of gliadin were affected by catechins. Esterified catechins tended to disrupt gliadin structures and non-esterified catechins polymerised gliadin molecules. Furthermore, molecular docking results indicated that catechins interacted with gliadin mainly by hydrogen bonds and hydrophobic action. The reactivity of catechins with gliadin was in the sequence as: epigallocatechin gallate > epicatechin gallate > epigallocatechin > epicatechin, which was based on the account of gallate and B-ring hydroxyl number discrepancy. All results suggested that catechins affected greatly on gliadin, and TPs were potentially used to improve the quality of flour products.

摘要

研究了茶多酚(TPs)对面条品质的影响,并探讨了儿茶素与麦谷蛋白之间的相互作用机制。随着 TPs 的添加,面条的理化性质和感官特性发生了显著变化。当 TPs 添加量为 0.5%时,面条的吸水率、拉伸强度和弹性分别增加了 1.35%、4.98%和 28.51%,然后随着 TPs 添加量的增加而降低。根据表面疏水性、空间结构、热性能、氨基和巯基含量的测定,儿茶素影响了麦谷蛋白的结构和性质。酯化儿茶素倾向于破坏麦谷蛋白的结构,而非酯化儿茶素则使麦谷蛋白分子聚合。此外,分子对接结果表明,儿茶素主要通过氢键和疏水作用与麦谷蛋白相互作用。儿茶素与麦谷蛋白的反应活性顺序为:没食子酸表儿茶素没食子酸> > 表儿茶素没食子酸> > 表儿茶素> > 表儿茶素,这是基于没食子酸和 B 环羟基数量差异的考虑。所有结果表明,儿茶素对麦谷蛋白有很大的影响,TPs 可能被用于改善面粉产品的质量。

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