Environment Research Institute, Shandong University, Qingdao, 266237, China.
Jiangsu Surveying and Design Institute of Water Resource Co.,Ltd, Yangzhou, 225127, China.
Environ Sci Pollut Res Int. 2022 Oct;29(47):71766-71773. doi: 10.1007/s11356-022-20103-6. Epub 2022 May 23.
Iron ore and manganese ore were used as substrate of constructed wetlands (CWs) to enhance nitrogen (N) removal. However, the N purification performance in CWs filled with iron or manganese ore under antibiotics stress needs further study. In this study, three groups of CWs filled with river sand, limonite (a kind of iron ore), and manganese ore sand were constructed, which were named as C-CWs, Fe-CWs, and Mn-CWs, respectively. The effect and mechanism of the composite antibiotics sulfamethoxazole (SMX) and trimethoprim (TMP) on N removal in CWs were investigated. While the addition of SMX and TMP inhibited about 40% nitrification and promoted about 25% denitrification in all CWs, Fe-CWs and Mn-CWs always had better N removal performance than C-CWs. Changes in microbial community structure in CWs indicated that the better N removal performance in Fe-CWs and Mn-CWs was attributed to the presence of more abundant and diverse N-associated bacteria, especially Fe- and Mn-driven autotrophic denitrifying bacteria. What's more, the addition of iron ore or manganese ore contributed to the better N removal performance with highest relative abundance of N-transferring bacteria under antibiotics stress.
铁锰矿被用作人工湿地（CWs）的基质以增强氮（N）去除。然而，在抗生素胁迫下，填充铁或锰矿的 CWs 的 N 净化性能仍需要进一步研究。本研究构建了三组分别填充河沙、针铁矿（一种铁矿）和锰矿砂的 CWs，分别命名为 C-CWs、Fe-CWs 和 Mn-CWs。研究了复合抗生素磺胺甲恶唑（SMX）和甲氧苄啶（TMP）对 CWs 中 N 去除的影响和作用机制。尽管 SMX 和 TMP 的添加抑制了所有 CWs 中约 40%的硝化作用，并促进了约 25%的反硝化作用，但 Fe-CWs 和 Mn-CWs 的 N 去除性能始终优于 C-CWs。CWs 中微生物群落结构的变化表明，Fe-CWs 和 Mn-CWs 中更丰富和多样的 N 相关细菌，特别是铁和锰驱动的自养脱氮细菌的存在，是其具有更好 N 去除性能的原因。此外，在抗生素胁迫下，添加铁锰矿有助于提高 N 去除性能，其具有更高的 N 转移细菌相对丰度。
