Dai Liang, Ng Siow Yee, Doyle Patrick S, van der Maarel Johan R C
BioSystems and Micromechanics, Singapore-MIT Alliance for Research and Technology, Singapore.
Department of Physics, National University of Singapore, Singapore 117542.
ACS Macro Lett. 2012 Aug 21;1(8):1046-1050. doi: 10.1021/mz300323a. Epub 2012 Aug 2.
Currently, no theory is available to describe the conformation of DNA confined in a channel when the nanochannel diameter is around the persistence length. Back-folded hairpins in the undulating wormlike chain conformation result in the formation of loops, which reduces the stretch of the molecule in the longitudinal direction of the channel. A cooperativity model is used to quantify the frequency and size of the loop domains. The predictions agree with results from the Monte Carlo simulation.
目前，当纳米通道直径约为持久长度时，尚无理论可描述限制在通道内的DNA构象。呈起伏状蠕虫状链构象的反向折叠发夹会导致环的形成，这会减少分子在通道纵向的伸展。采用协同模型来量化环域的频率和大小。这些预测与蒙特卡罗模拟的结果一致。
