Research Laboratory of Spectrometry & Micro and Nano Extraction, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, Iran.
ACS Biomater Sci Eng. 2022 Jun 13;8(6):2477-2488. doi: 10.1021/acsbiomaterials.2c00068. Epub 2022 May 24.
Cancer is one of the most difficult diseases to treat, threatening the lives of millions of people today. So far, various methods have been used to treat cancer, each having its drawbacks. One of these methods is treatment with anticancer drugs, which unfortunately have severe side effects. One of the causes of these complications is the nonspecific effects of anticancer drugs, which attack normal cells in addition to cancer cells and damage healthy tissues. In this study, we are trying to reduce the side effects and increase the efficacy of the drug by providing smart drug delivery. The metal-organic framework (MOF) was rapidly synthesized using a microwave method and at the nanoscale. The particle size of NMOF-5 was 18-20 nm, and its surface area was 2690 m·g. A chitosan polymer coating was formed on the nanocarrier after 6-mercaptopurine was introduced. The biocompatible nanocarrier exhibited a high capacity to adsorb the drug. The biocompatible nanocarrier slowly and uniformly released 96.78% of the drug in a simulated solution at pH 5 and 20.52% at pH 7.4. This showed that CS-6-MP-NMOF-5 released the drug smartly and pH-sensitively. The stability of the biocompatible nanocarrier was studied at different pH values and remained stable at pH 5 for up to 48 h. The toxicity study of the MCF-7 cell line at different concentrations for 24 h showed the excellent performance of the biocompatible nanocarrier compared to the free drug in terms of toxicity to breast cancer cells.
癌症是最难治疗的疾病之一，威胁着当今数百万人的生命。到目前为止，已经使用了各种方法来治疗癌症，每种方法都有其缺点。其中一种方法是使用抗癌药物治疗，不幸的是，这些药物有严重的副作用。这些并发症的原因之一是抗癌药物的非特异性作用，除了癌细胞外，它还会攻击正常细胞并损害健康组织。在这项研究中，我们试图通过提供智能药物递送来减少副作用并提高药物的疗效。金属有机骨架（MOF）使用微波方法在纳米尺度上快速合成。NMOF-5 的粒径为 18-20nm，比表面积为 2690m·g。在引入 6-巯基嘌呤后，在纳米载体上形成壳聚糖聚合物涂层。生物相容的纳米载体表现出高吸附药物的能力。在 pH5 和 20.52%的 pH7.4 模拟溶液中，生物相容的纳米载体缓慢且均匀地释放了 96.78%的药物。这表明 CS-6-MP-NMOF-5 可以智能且对 pH 敏感地释放药物。在不同 pH 值下研究了生物相容的纳米载体的稳定性，在 pH5 下稳定长达 48 小时。不同浓度 MCF-7 细胞系 24 小时的毒性研究表明，与游离药物相比，生物相容的纳米载体对乳腺癌细胞的毒性具有优异的性能。
