Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China.
Jiangsu University of Science and Technology, Zhenjiang 212018, Jiangsu Province, China; Sericulture Research Institute of Chinese Academy of Agricultural Sciences, Zhenjiang 212018, Jiangsu Province, China.
Gene. 2022 Aug 5;834:146607. doi: 10.1016/j.gene.2022.146607. Epub 2022 May 21.
As a typical species of microsporidium, Nosema bombycis is the pathogen causing the pébrine disease of silkworm. Rapid proliferation of N. bombycis in host cells requires replication of genetic material. As eukaryotic origin recognition protein, origin recognition complex (ORC) plays an important role in regulating DNA replication, and Orc1 is a key subunit of the origin recognition complex. In this study, we identified the Orc1 in the microsporidian N. bombycis (NbOrc1) for the first time. The NbOrc1 gene contains a complete ORF of 987 bp in length that encodes a 328 amino acid polypeptide. Indirect immunofluorescence results showed that NbOrc1 were colocalized with Nbactin and NbSAS-6 in the nuclei of N. bombycis. Subsequently, we further identified the interaction between the NbOrc1 and Nbactin by CO-IP and Western blot. These results imply that Orc1 may be involved in the proliferation of the microsporidian N. bombycis through interacting with actin.
作为微孢子虫的典型物种，蚕微孢子虫是引起家蚕微粒子病的病原体。微孢子虫在宿主细胞内的快速增殖需要遗传物质的复制。作为真核生物的起始识别蛋白，起始识别复合物（ORC）在调节 DNA 复制中起着重要作用，而 Orc1 是起始识别复合物的关键亚基。在本研究中，我们首次鉴定了微孢子虫蚕微孢子虫（NbOrc1）中的 Orc1。NbOrc1 基因包含一个完整的 987bp 的 ORF，编码一个 328 个氨基酸的多肽。间接免疫荧光结果显示，NbOrc1 与 Nbactin 和 NbSAS-6 在蚕微孢子虫的细胞核中共定位。随后，我们通过 CO-IP 和 Western blot 进一步鉴定了 NbOrc1 与 Nbactin 之间的相互作用。这些结果表明，Orc1 可能通过与肌动蛋白相互作用参与微孢子虫 N. bombycis 的增殖。
