Agricultural Biotechnology Department, Graduate School of Natural and Applied Sciences, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Agricultural Biotechnology Department, Faculty of Agriculture, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Mol Biol Rep. 2022 Jun;49(6):5659-5668. doi: 10.1007/s11033-022-07584-x. Epub 2022 May 25.
Onobrychis viciifolia Scop. is a short-lived perennial cool-season legume used for forage production. It is a common native species in Asia Minor, especially in Turkey, the districts of the Caucasus, and the Caspian fringes. It can grow well in a broad range of climatic and soil types found in Asia, Europe, and North America. It is a non-bloating crop, making it suitable for use in both hay and pasture.
The aim was to assess the diversity of the 83 sainfoin genotypes selected based on their high agronomic performance from a germplasm collection evaluated in the experimental field of Tekirdag Namik Kemal University, Turkey. Ten nuclear simple sequence repeat (nSSR) primers (OVK036, OVK046, OVK094, OVK101, OVK125, OVK161, OVK174, OVM033, OVM061, and OVM125) were used in the study. All nSSR loci were found to be polymorphic and totally 92 alleles were detected. The mean observed number of alleles per locus was calculated as 9.2. Among the genetic diversity parameters, Shannon Index (I = 0.375), unbiased genetic diversity value (uh = 0.243), and mean polymorphic information content (PIC = 0.240) were calculated. The genetic distance value varied between 0.43 and 0.95. Based on the dendrogram built by the UPGMA clustering method using genetic distance values, it was observed that the studied sainfoin genotypes were divided into two main clusters, whereas the STRUCTURE analysis results had high support for three clusters.
The results obtained from this study provide important information on the genetic structures of the studied sainfoin genotypes and their genetic relationship. Therefore acquired genetic data will be useful in designing more efficient polycross nurseries, allowing open pollination of best performing and genetically diverse genotypes in the isolated conditions, which will increase genetic gain in sainfoin breeding programs.
苦马豆是一种短寿命的多年生豆科冷季牧草，用于饲料生产。它是亚洲小亚细亚的一种常见本地物种，特别是在土耳其、高加索地区和里海边缘。它可以在亚洲、欧洲和北美的广泛气候和土壤类型中生长良好。它是一种不膨气的作物，因此适合用于干草和牧场。
本研究旨在评估从土耳其 Tekirdag Namik Kemal 大学实验田评估的种质资源中选择的 83 个苦马豆基因型的多样性，这些基因型基于其高农艺表现。使用了 10 个核简单序列重复（nSSR）引物（OVK036、OVK046、OVK094、OVK101、OVK125、OVK161、OVK174、OVM033、OVM061 和 OVM125）。所有 nSSR 位点均表现出多态性，共检测到 92 个等位基因。每个位点的平均观察等位基因数计算为 9.2。在遗传多样性参数中，计算了香农指数（I=0.375）、无偏遗传多样性值（uh=0.243）和平均多态信息含量（PIC=0.240）。遗传距离值在 0.43 到 0.95 之间变化。基于基于遗传距离值构建的 UPGMA 聚类方法的聚类图，观察到所研究的苦马豆基因型分为两个主要聚类，而 STRUCTURE 分析结果强烈支持三个聚类。
本研究结果提供了有关所研究苦马豆基因型遗传结构及其遗传关系的重要信息。因此，获得的遗传数据将有助于设计更有效的杂交苗圃，允许在隔离条件下对表现最好和遗传多样性最好的基因型进行开放授粉，从而提高苦马豆育种计划的遗传增益。
