Gontijo Filho P P, Wayne L G
Rev Bras Pesqui Med Biol. 1978 Jun;11(2-3):117-25.
The biological activity of rabbit peritoneal and alveolar macrophages activated with M. bovis BCG was investigated by use of tests measuring their phagocytic and staphylocidal properties. Macrophages were separated in a discontinuous albumin gradient which yielded 4 to 5 bands depending on the source of the exudate. The peritoneal exudate had about 40% of cells recovered in band E and the alveolar exudate had about 35% in band C. There were not significant differences between the uptake by three subpopulations of macrophages. The alveolar macrophages from normal rabbits were less active than peritoneal macrophages. They were also less active those from vaccinated animals. Subpopulations of band B macrophages killed S. aureus more efficient than those from band C. Such differences reflect the number of mature or immature cells in each subpopulations. Mature cells (bond B and C) possess phagocytic and bactericidal activities whereas immature cells (bond D) are capable of ingesting bacteria but no killing them.
通过检测兔腹膜和肺泡巨噬细胞的吞噬及杀葡萄球菌特性试验，研究了用卡介苗（M. bovis BCG）激活的兔腹膜和肺泡巨噬细胞的生物活性。巨噬细胞在不连续白蛋白梯度中分离，根据渗出液来源可产生4至5条带。腹膜渗出液约40%的细胞在E带回收，肺泡渗出液约35%的细胞在C带回收。巨噬细胞三个亚群的摄取之间无显著差异。正常兔的肺泡巨噬细胞比腹膜巨噬细胞活性低。它们也比接种动物的肺泡巨噬细胞活性低。B带巨噬细胞亚群比C带巨噬细胞亚群更有效地杀死金黄色葡萄球菌。这种差异反映了每个亚群中成熟或未成熟细胞的数量。成熟细胞（B带和C带）具有吞噬和杀菌活性，而未成熟细胞（D带）能够摄取细菌但不能杀死它们。
