天然成分栀子苷可提高感染白斑综合征病毒的克氏原螯虾的存活率。
Natural component geniposide enhances survival rate of crayfish Procambarus clarkii infected with white spot syndrome virus.
机构信息
Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China; College of Life Science and Technology, Guangxi University, Nanning, 530004, China.
Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Guangxi University, Nanning, 530004, China; School of Marine Sciences, Guangxi University, Nanning, 530004, China.
出版信息
Fish Shellfish Immunol. 2022 Jul;126:96-103. doi: 10.1016/j.fsi.2022.05.037. Epub 2022 May 22.
White Spot Disease (WSD), caused by white spot syndrome virus (WSSV), is an acute and highly lethal viral disease of shrimp. Currently, there are no commercially available drugs to control WSD. It is urgent and necessary to find anti-WSSV drugs. Natural compounds are an important source of antiviral drug discovery. In this study, the anti-WSSV activity of natural compound geniposide (GP) was investigated in crayfish Procambarus clarkii. Results showed that GP had a concentration-dependent inhibitory effect on WSSV replication in crayfish at 24 h, and highest inhibition was more than 98%. In addition, GP significantly inhibited the expression of WSSV immediate-early gene ie1, early gene DNApol, late gene VP28. The mortality of WSSV-infected crayfish in control groups was 100%, while it reduced by 70.0% when treated with 50 mg/kg GP. Co-incubation, pre-treatment and post-treatment experiments showed that GP could prevent and treat WSSV infection in crayfish by significantly inhibiting WSSV multiplication. Mechanistically, the syntheses of WSSV structural proteins VP19, VP24, VP26 and VP28 were significantly inhibited by GP in S2 cells. Furthermore, GP could also suppress WSSV replication by blocking the expression of antiviral immunity-related factor STAT to reduce ie1 transcription. Moreover, GP possessed anti-inflammatory and anti-oxidative activity in crayfish. Overall, GP has the potential to be developed as a preventive or therapeutic agent against WSSV infection.
白斑综合征（WSD）是由白斑综合征病毒（WSSV）引起的虾类急性、高致死性病毒性疾病。目前，尚无市售药物可用于控制 WSD。寻找抗 WSSV 药物迫在眉睫，十分必要。天然化合物是抗病毒药物发现的重要来源。本研究以克氏原螯虾为研究对象，探讨了天然化合物京尼平苷（GP）对 WSSV 的抗活性。结果表明，GP 对虾体内 WSSV 的复制具有浓度依赖性抑制作用，24 h 时最高抑制率超过 98%。此外，GP 还显著抑制了 WSSV 即刻早期基因 ie1、早期基因 DNApol、晚期基因 VP28 的表达。对照组感染 WSSV 的虾死亡率为 100%，而用 50mg/kg GP 处理后死亡率降低了 70.0%。共孵育、预处理和后处理实验表明，GP 可通过显著抑制 WSSV 增殖来预防和治疗虾类 WSSV 感染。在机制上，GP 可显著抑制 S2 细胞中 WSSV 结构蛋白 VP19、VP24、VP26 和 VP28 的合成。此外，GP 还可以通过抑制抗病毒免疫相关因子 STAT 的表达来抑制 WSSV 复制，从而减少 ie1 的转录。此外，GP 在虾体内还具有抗炎和抗氧化活性。综上所述，GP 具有作为预防或治疗 WSSV 感染的候选药物的潜力。
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