Dong Wei, Liang Erli, Zhang Qiong, Li Rui, Wang Yunfang, Liu Jianxin, Zhang Xiaochao, Wang Yawen, Fan Caimei
College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, Shanxi, China.
Photochem Photobiol. 2023 Jan;99(1):83-91. doi: 10.1111/php.13657. Epub 2022 Jun 6.
In this study, a set of BiOCl with controllable ratios of (001) and (110) facets was prepared by adjusting the content of diethylene glycol (DEG) during the preparation process. The degradation experiment of bisphenol A (BPA) shows that under simulated sunlight, when the ratio of (001) to (110) is 0.61, BiOCl (BOC-2) has the best degradation activity, which can degrade 96.2% BPA within 20 min. After theoretical calculations and experimental characterization, a Z-scheme (001)/(110) facet homojunction is proposed. Then, three typical samples were selected to test the biological toxicity of HepG2 cells and the activity of killing HepG2 cells under ultraviolet light conditions. Studies have found that exposed facets play a more important role in the biotoxicity of BiOCl to cells; with a (001)/(110) ratio of 0.61, BOC-2 exhibits excellent endocytosis and phototoxicity but no obvious dark cytotoxicity, while with a (001)/(110) ratio of 0.15, BiOCl (BOC-4) has poor endocytosis and strong cytotoxicity under dark conditions. Through reactive oxygen species (ROS) and lactate dehydrogenase (LDH) assay detection, the process of photocatalytic killing cells of BOC-2 more looks like an apoptosis mechanism, while BOC-4 mainly causes cell necrosis.
在本研究中，通过在制备过程中调节二甘醇（DEG）的含量，制备了一组具有可控（001）和（110）面比例的BiOCl。双酚A（BPA）的降解实验表明，在模拟太阳光下，当（001）与（110）的比例为0.61时，BiOCl（BOC-2）具有最佳的降解活性，在20分钟内可降解96.2%的BPA。经过理论计算和实验表征，提出了一种Z型（001）/（110）面同质结。然后，选择三个典型样品测试HepG2细胞的生物毒性以及在紫外光条件下杀死HepG2细胞的活性。研究发现，暴露的晶面对BiOCl对细胞的生物毒性起更重要的作用；当（001）/（110）比例为0.61时，BOC-2表现出优异的内吞作用和光毒性，但无明显的暗细胞毒性，而当（001）/（110）比例为0.15时，BiOCl（BOC-4）在黑暗条件下内吞作用较差且具有较强的细胞毒性。通过活性氧（ROS）和乳酸脱氢酶（LDH）检测分析，BOC-2光催化杀死细胞的过程更像是一种凋亡机制，而BOC-4主要导致细胞坏死。
