Department of Chemistry, University of British Columbia, Vancouver, British Columbia, Canada; Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada.
Institute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, Québec, Canada.
Biophys J. 2022 Jun 21;121(12):2290-2296. doi: 10.1016/j.bpj.2022.05.026. Epub 2022 May 25.
The matrix proteins (M) of many enveloped RNA viruses mediate virus assembly and budding. However, it remains poorly understood how M are involved in virus budding and how they interact with envelope proteins. Here, we show that the expression level of Nipah (NiV) M in particles produced by the host cells deviates from a gamma distribution and does not reflect that of the host cells, indicating assembly of the NiV-M in the process. Our data reveal that NiV-M affects the circularity of the particles while the NiV envelope proteins do not. The organization of NiV envelope proteins on the membrane of the particles is similar to those that do not express NiV-M, suggesting that NiV-M does not directly interact with the envelope proteins during assembly and budding.
许多包膜 RNA 病毒的基质蛋白（M）介导病毒的组装和出芽。然而，M 如何参与病毒出芽以及它们如何与包膜蛋白相互作用仍知之甚少。在这里，我们表明，宿主细胞产生的颗粒中的尼帕病毒（NiV）M 的表达水平偏离了伽马分布，并且不反映宿主细胞的表达水平，表明 NiV-M 在该过程中进行组装。我们的数据表明 NiV-M 影响颗粒的圆度，而 NiV 包膜蛋白则不影响。颗粒膜上 NiV 包膜蛋白的组织类似于不表达 NiV-M 的那些，表明 NiV-M 在组装和出芽过程中不与包膜蛋白直接相互作用。
