Ma Xiaoqian, Lu Xinming, Zhang Ping, Deng Xun, Bai Jianyang, Xu Zhe, Diao Jian, Pang Hongyang, Wang Qi, Zhao Hongying, Ma Wei, Ma Ling
College of Forestry, Northeast Forestry University, Harbin, China.
Forest Protection Research Institute, HeiLongJiang Academy of Forestry, Harbin, China.
Front Physiol. 2022 May 9;13:896793. doi: 10.3389/fphys.2022.896793. eCollection 2022.
The olfactory system plays a key role in regulating insect behaviors, such as locating host plants, spawning sites, and mating partners and avoiding predators. Chemosensory genes are required for olfactory recognition in insects. Faust. (Coleoptera: Curculionidae) damages hazelnuts and causes severe economic losses. There are no effective control measures, but understanding the olfaction mechanisms of this insect could lead to a new approach for population management. However, the genes that perform chemosensory functions in are still unclear. Using high-throughput sequencing, we assembled the antennal transcriptome of and annotated the major chemosensory gene families. Of the chemosensory gene families, we found 23 odorant-binding proteins, 15 chemosensory proteins, 2 sensory neuron membrane proteins, 15 odorant receptors, 23 ionotropic receptors, and nine gustatory receptors. Using Blast sequence alignment and phylogenetic analysis, the sequences of these proteins were identified. Male- and female-specific chemosensory genes involved in odorant detection and recognition were validated by qRT-PCR. Among the chemosensory genes, we found significant differences in the expression of CdieOBP8, CdieOBP9, CdieOBP19, CdieOBP20, CdieOBP21, CdieCSP15, CdieOR13, and CdieOR15 between adult male and female . A total of 87 expressed chemosensory proteins were found in . Investigating these proteins will help reveal the molecular mechanism of odorant recognition in and may aid the development of novel control strategies for this species.
嗅觉系统在调节昆虫行为方面起着关键作用，例如定位寄主植物、产卵地点和交配伙伴以及躲避捕食者。化学感应基因是昆虫嗅觉识别所必需的。法氏象甲（鞘翅目：象甲科）会损害榛子并造成严重的经济损失。目前尚无有效的控制措施，但了解这种昆虫的嗅觉机制可能会带来一种新的种群管理方法。然而，法氏象甲中执行化学感应功能的基因仍不清楚。我们利用高通量测序技术组装了法氏象甲的触角转录组，并注释了主要的化学感应基因家族。在化学感应基因家族中，我们发现了23种气味结合蛋白、15种化学感应蛋白、2种感觉神经元膜蛋白、15种气味受体、23种离子otropic受体和9种味觉受体。通过Blast序列比对和系统发育分析，鉴定了这些蛋白质的序列。通过qRT-PCR验证了参与气味检测和识别的雄性和雌性特异性化学感应基因。在化学感应基因中，我们发现法氏象甲成年雄性和雌性之间CdieOBP8、CdieOBP9、CdieOBP19、CdieOBP20、CdieOBP21、CdieCSP15、CdieOR13和CdieOR15的表达存在显著差异。在法氏象甲中总共发现了87种表达的化学感应蛋白。对这些蛋白质的研究将有助于揭示法氏象甲气味识别的分子机制，并可能有助于开发针对该物种的新型控制策略。
