School of Marine Sciences, Sun Yat-Sen University, Guangzhou, Guangdong 510275, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, Guangdong 519000, China.
Zool Res. 2022 Jul 18;43(4):537-551. doi: 10.24272/j.issn.2095-8137.2022.058.
production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture, especially in fish with relatively long generations. Nevertheless, functional sperm production from -cultured spermatogonia remains a challenge in most aquaculture fish. In this study, we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers ( ), which are prone to ovotesticular or sterile testicular development, and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional (3D) culture system. Artificial insemination indicated that the -derived sperm were capable of fertilizing mature oocytes to develop into normal larvae. Furthermore, melatonin significantly promoted spermatogonia proliferation and differentiation through the ERK1/2 signaling pathway, and thus increased the efficiency in functional sperm production. The 3D culture system and resulting functional sperm hold great promise for improving the genetic breeding of aquaculture fish.
生产功能性配子可以通过减少世代间隔和加速水产养殖中的遗传育种来彻底改变繁殖，尤其是在世代相对较长的鱼类中。然而，从培养的精原细胞中产生功能性精子仍然是大多数水产养殖鱼类面临的挑战。在这项研究中，我们从易于发生卵睾或不育睾丸发育的海洋四眼睡鲨中分离并鉴定了减数分裂前的精原细胞，并在三维（3D）培养系统中诱导精原细胞分化为有鞭毛的精子。人工授精表明，-衍生的精子能够使成熟卵子受精并发育成正常幼虫。此外，褪黑素通过 ERK1/2 信号通路显著促进精原细胞增殖和分化，从而提高了功能性精子生产的效率。3D 培养系统和功能性精子的产生为改善水产养殖鱼类的遗传育种带来了巨大的希望。
