Moubarz Gehan, Shousha Wafaa G, Saad-Hussein Amal, Shawky Michael M, Shawky Shiamaa
Environmental & Occupational Medicine Department, Environment & Climate Change Research Institute, National Research Centre, Cairo, Egypt.
Faculty of Science, Helwan University, Cairo, Egypt.
Int J Environ Health Res. 2023 Dec;33(12):1195-1204. doi: 10.1080/09603123.2022.2080189. Epub 2022 May 26.
Chitotriosidase (CHIT1) is involved in the innate defense against chitin-containing pathogens. In the present study, we sought to investigate the role of CHIT1 gene polymorphisms on susceptibility to infection in addition to oxidative stress caused by infection. CHIT1 gene polymorphisms were identified in 60 -positive workers by REFLP. We also measured concentrations of the CHIT1 enzyme, total antioxidant capacity (TAC), and malondialdehyde (MDA). The majority of workers were wild-type (AA) (66.5%), followed by heterozygous (AB) (28.5%), and homozygous mutants (BB) (5%). The mean concentrations of specific IgE for all species were affected by change in CHIT1 genotypes. Our findings indicate that decreased CHIT1 activity in homozygous mutant CHIT1 allele is associated with a subsequent decrease in TAC levels, resulting in an increased risk of fungal infection and accumulation of oxidant MDA. Thus, CHIT1 enzyme activity plays a critical role in the susceptibility of WWTP workers to fungal infections.
壳三糖苷酶（CHIT1）参与针对含几丁质病原体的天然防御。在本研究中，我们试图除了研究感染引起的氧化应激外，还探讨CHIT1基因多态性在感染易感性中的作用。通过限制性片段长度多态性（REFLP）在60名阳性工作者中鉴定出CHIT1基因多态性。我们还测量了CHIT1酶的浓度、总抗氧化能力（TAC）和丙二醛（MDA）。大多数工作者为野生型（AA）（66.5%），其次是杂合子（AB）（28.5%），以及纯合突变体（BB）（5%）。所有物种特异性IgE的平均浓度受CHIT1基因型变化的影响。我们的研究结果表明，纯合突变CHIT1等位基因中CHIT1活性降低与随后TAC水平降低相关，导致真菌感染风险增加和氧化剂MDA积累。因此，CHIT1酶活性在污水处理厂工作者对真菌感染的易感性中起关键作用。
