Department of Cardiovascular Diseases, Civil Aviation General Hospital, Peking University, Beijing, China.
School of Life and Pharmaceutical Sciences, Dalian University of Technology, Panjin, China.
Acta Biochim Pol. 2022 May 25;69(2):399-407. doi: 10.18388/abp.2020_5851.
Lipid droplets (LDs) are intracellular droplets containing phospholipids and neutral lipids. It is well known that LDs are organelles with a rich proteome. In the nervous system, these droplets may play an important role in maintaining the normal physiological function of nerve cells. Moreover, LDs may relate to the neurodegenerative disorders, such as Alzheimer's disease (AD). However, more information is still needed about the function of LDs. In the study presented here, we identified the protein composition of mouse neuroblastoma (N2a) cell LDs using immunodetection and high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS). Seventy three LDs proteins were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to investigate the potential functions of these proteins. Subsequently, the relationships among the proteins were analyzed by constructing a protein-protein interaction (PPI) network. N2a cell LDs contain multiple Rab GTPases, chaperones, and proteins involved in ubiquitination and transport. Some of these proteins were known to modulate LD formation and were related to the function of nerve cells. This work presents the proteome of N2a cell LDs and will help to identify the role of LDs in the nervous system.
脂滴（LDs）是含有磷脂和中性脂质的细胞内液滴。众所周知，LDs 是一种富含蛋白质组的细胞器。在神经系统中，这些液滴可能在维持神经细胞的正常生理功能方面发挥重要作用。此外，LDs 可能与神经退行性疾病有关，如阿尔茨海默病（AD）。然而，关于 LDs 的功能仍需要更多的信息。在本研究中，我们使用免疫检测和高效液相色谱（HPLC）与质谱（MS）联用的方法鉴定了小鼠神经母细胞瘤（N2a）细胞 LDs 的蛋白质组成。鉴定出 73 种 LDs 蛋白。进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析，以研究这些蛋白质的潜在功能。随后，通过构建蛋白质-蛋白质相互作用（PPI）网络分析蛋白质之间的关系。N2a 细胞 LDs 包含多种 Rab GTPases、伴侣蛋白以及参与泛素化和运输的蛋白。其中一些蛋白已知可调节 LD 的形成，并与神经细胞的功能有关。这项工作展示了 N2a 细胞 LDs 的蛋白质组，将有助于确定 LDs 在神经系统中的作用。
