Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany.
Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany.
Methods Mol Biol. 2022;2480:127-145. doi: 10.1007/978-1-0716-2241-4_9.
Recombinant proteins produced in whole plants are usually extracted and purified from leaf or seed tissues. Here we describe a workflow that is suitable for most soluble pharmaceutical proteins produced in leafy biomass. We highlight options to streamline the process, reducing the total process time and costs, and eliminating certain unit operations if the product allows. The overall process is a combination of batch-wise or continuous extraction followed by a series of solid-liquid separation steps, which can be supported by chemically defined additives, and concludes with a sequence of optional membrane-based and regular chromatographic purification operations.
在全植物中生产的重组蛋白通常从叶片或种子组织中提取和纯化。在这里，我们描述了一种适用于大多数在叶状生物质中生产的可溶性药物蛋白的工作流程。我们强调了简化流程的选择，如果产品允许，可以减少总处理时间和成本，并消除某些单元操作。整个过程是分批或连续提取的组合，然后是一系列固液分离步骤，可以通过化学定义的添加剂来支持，最后是一系列可选的基于膜的和常规的色谱纯化操作。
