Ghosh Subhabrata, Hollingsworth Jennifer A, Gallea Jose Ignacio, Majumder Somak, Enderlein Jörg, Chizhik Alexey I
Third Institute of Physics-Biophysics, University of Göttingen, Friedrich-Hund-Platz 1, D-37077 Göttingen, Germany.
Center for Integrated Nanotechnologies, Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, NM 87545, United States of America.
Nanotechnology. 2022 Jun 15;33(36). doi: 10.1088/1361-6528/ac73a2.
We report on proof of principle measurements of a concept for a super-resolution imaging method that is based on excitation field density-dependent lifetime modulation of semiconductor nanocrystals. The prerequisite of the technique is access to semiconductor nanocrystals with emission lifetimes that depend on the excitation intensity. Experimentally, the method requires a confocal microscope with fluorescence-lifetime measurement capability that makes it easily accessible to a broad optical imaging community. We demonstrate with single particle imaging that the method allows one to achieve a spatial resolution of the order of several tens of nanometers at moderate fluorescence excitation intensity.
我们报告了一种基于半导体纳米晶体激发场密度依赖寿命调制的超分辨率成像方法概念的原理验证测量。该技术的前提是能够获得发射寿命取决于激发强度的半导体纳米晶体。在实验上，该方法需要一台具备荧光寿命测量能力的共聚焦显微镜，这使得广大光学成像领域的人员都能轻易使用。我们通过单粒子成像证明，该方法能够在中等荧光激发强度下实现几十纳米量级的空间分辨率。
