基于异佛尔酮的半胱氨酸快速近红外荧光探针及其在 B16 细胞成像中的应用。
A Rapid Near-Infrared Fluorescent Probe for Cysteine Based on Isophorone and its Application in B16 Cell Imaging.
机构信息
School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
Department of Neurology, The Third People's Hospital of Chengdu, the Affiliated Hospital of Southwest Jiaotong University, Chengdu, 610031, People's Republic of China.
出版信息
J Fluoresc. 2022 Sep;32(5):1661-1667. doi: 10.1007/s10895-022-02964-9. Epub 2022 May 26.
A novel near-infrared fluorescent probe SWJT-5 based on dicyanoisophorone was synthesized. It achieved the rapid (within 40 s) and discriminative detection of Cys over Hcy and GSH with a large Stokes shift (205 nm). It showed high selectivity and sensitivity for Cys, and had an obvious enhancement of fluorescence emission. The detection limit was 0.43 μM. This probe also had low background interference and little damage to biological samples. Therefore, SWJT-5 had been applied to bioimaging in living cells successfully.
基于二氰基异佛尔酮合成了一种新型近红外荧光探针 SWJT-5。它实现了对 Cys 与 Hcy 和 GSH 的快速（40s 内）和区分检测，Stokes 位移大（205nm）。它对 Cys 表现出高选择性和灵敏度，并且荧光发射明显增强。检测限为 0.43μM。该探针还具有低背景干扰和对生物样品损伤小的特点。因此，SWJT-5 已成功应用于活细胞的生物成像。
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