Institute of Pharmacology and Toxicology, University Medical Center Göttingen, Göttingen, Germany.
DZHK (German Center for Cardiovascular Research), Partner Site Göttingen, Göttingen, Germany.
Methods Mol Biol. 2022;2485:213-225. doi: 10.1007/978-1-0716-2261-2_14.
Different engineered heart muscle formats have been developed for applications in disease modeling, drug screening, and heart repair. The advantage of 3D engineered versus 2D monolayer and 3D aggregate cardiomyocyte cultures is a clearly advanced degree of maturation, which in many aspects resembles the postnatal rather than the embryonic or fetal heart, in the most advanced 3D culture formats. According to the desired in vitro (disease modeling or drug screening) and in vivo (heart repair) application, scale and geometry of tissue engineered heart muscle must be adapted. In this updated methods paper, we report a simple and scalable (up and down) collagen-based protocol for the construction of Engineered Human Myocardium (EHM) under defined, serum-free conditions.
不同的工程化心肌细胞形式已被开发用于疾病建模、药物筛选和心脏修复。与 2D 单层和 3D 聚集心肌细胞培养相比，3D 工程化的优势在于明显更高的成熟度，在最先进的 3D 培养形式中，在许多方面类似于出生后的心脏，而不是胚胎或胎儿心脏。根据体外（疾病建模或药物筛选）和体内（心脏修复）应用的需要，组织工程化心肌的规模和几何形状必须进行调整。在本更新的方法论文中，我们报告了一种简单且可扩展（向上和向下）的基于胶原蛋白的方案，用于在无血清条件下构建工程化人类心肌（EHM）。
