Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.
Center for Advanced Medical and Life Science, Tokyo Women's Medical University, Tokyo, Japan.
Methods Mol Biol. 2022;2485:227-242. doi: 10.1007/978-1-0716-2261-2_15.
This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of small intestine, which functions as a blood vessel bed. The capillaries of the small intestinal segment are fed by an artery and drained by a vein. Therefore, perfusion culture of the cardiac tissue is achieved by continuously infusing culture medium into the arterial vessel that supplies the segment of small intestine. The aim of this technique is to fabricate tubular cardiac tissue that can function as a pump by sequentially implanting and culturing cardiac cell sheets on the inner wall of a perfused segment of small intestine.
本章介绍了一种体外构建管状心脏组织的方法。通过将细胞片逐层堆叠在小段小肠的内壁上，形成管状结构，可以制备出厚的心脏组织。小段小肠的毛细血管由一条动脉供应，由一条静脉引流。因此，通过持续向供应小肠段的动脉灌注培养液，可以实现心脏组织的灌注培养。该技术的目的是通过在灌注的小肠段内壁上依次植入和培养心脏细胞片来构建可作为泵的管状心脏组织。
