National Medical Research Center of Hematology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2022 May;173(1):128-132. doi: 10.1007/s10517-022-05507-1. Epub 2022 May 27.
Multipotent mesenchymal stromal cells (MSC) were administered to patients after allogeneic hematopoietic stem cell transplantation to prevent the development of acute graft-versus- host disease (GVHD). The injection of MSC did not always prevent the development of GVHD. The aim of the work was to compare the secretome of MSC effective and ineffective in the prevention of GVHD. MSC were obtained from the bone marrow of hematopoietic stem cells donors. The secretome was studied using a TripleTOF 5600+ mass spectrometer with a NanoSpray III ion source coupled to a NanoLC Ultra 2D Plus nano-HPLC System. A total of 1,965 proteins were analyzed. Analysis of the secretome of effective and ineffective MSC samples revealed significant differences in the secretion of 1,119 proteins associated with ribosomes, exosomes, focal contacts, and others. Analysis of proteins secreted by MSC can be used to identify prognostically effective samples.
多能间充质干细胞（MSC）在异基因造血干细胞移植后被给予患者，以预防急性移植物抗宿主病（GVHD）的发生。MSC 的注射并不总是能预防 GVHD 的发生。本工作旨在比较 MSC 在预防 GVHD 方面有效和无效的分泌组。MSC 从造血干细胞供体的骨髓中获得。使用 TripleTOF 5600+质谱仪和与 NanoLC Ultra 2D Plus 纳流 HPLC 系统耦合的 NanoSpray III 离子源研究分泌组。共分析了 1965 种蛋白质。对有效和无效 MSC 样本分泌组的分析显示，与核糖体、外泌体、焦点接触等相关的 1119 种蛋白质的分泌存在显著差异。分析 MSC 分泌的蛋白质可用于鉴定预后有效的样本。
