College of Pharmacy, Idaho State University, Pocatello, ID, USA.
Institute of Arthritis Research LLC, Idaho Falls, ID, USA.
Inflammopharmacology. 2022 Aug;30(4):1295-1302. doi: 10.1007/s10787-022-01008-9. Epub 2022 May 26.
This study aimed to explore a correlation between plasma angiotensin II/(1-7) (Ang II/Ang-(1-7)) ratio, anti-ACE2 autoantibodies level and disease activity in rheumatoid arthritis (RA) patients.
In a pilot study, the plasma level of Ang II, Ang-(1-7), and anti-ACE2 autoantibodies of twelve RA patients (five in active stage and seven in remission) were measured using an LC-MS/MS method and an ELISA kit, respectively.
The Ang-(1-7) level was significantly higher in the remission group than in the active RA patients (7.63 ± 2.61 vs. 1.29 ± 0.81 ng/mL). On the contrary, the Ang II level was higher in those with active RA compared to the remission group (5.43 ± 1.82 vs. 0.87 ± 0.16 ng/mL). The mean ELISA score of anti-ACE2 autoantibodies in patients with active RA was significantly higher than patients in remission (1.41 ± 0.11 vs. 1.81 ± 0.11, p < 0.05).
This study result suggests that the angiotensin peptides concentration and anti-ACE2 autoantibodies levels can be used as biomarkers of RA. This will help clinicians evaluate better treatment success rates and disease prognosis to prevent long-term complications of RA.
本研究旨在探讨类风湿关节炎（RA）患者血浆血管紧张素 II/（1-7）（Ang II/Ang-（1-7））比值与抗 ACE2 自身抗体水平与疾病活动度之间的相关性。
在一项初步研究中，使用 LC-MS/MS 方法和 ELISA 试剂盒分别测量了 12 例 RA 患者（活动期 5 例，缓解期 7 例）的血浆 Ang II、Ang-（1-7）和抗 ACE2 自身抗体水平。
缓解期患者的 Ang-（1-7）水平明显高于活动期 RA 患者（7.63±2.61 vs. 1.29±0.81 ng/mL）。相反，活动期 RA 患者的 Ang II 水平高于缓解期患者（5.43±1.82 vs. 0.87±0.16 ng/mL）。活动期 RA 患者的抗 ACE2 自身抗体 ELISA 评分均值明显高于缓解期患者（1.41±0.11 vs. 1.81±0.11，p<0.05）。
本研究结果表明，血管紧张素肽浓度和抗 ACE2 自身抗体水平可作为 RA 的生物标志物。这将有助于临床医生更好地评估治疗成功率和疾病预后，以预防 RA 的长期并发症。
