Abteilung Mikrobiologie and CellNanOs-Center of Cellular Nanoanalytics Osnabrück, Fachbereich Biologie/Chemie, Universität Osnabrück Barbarastr, Osnabrück, Germany.
Methods Mol Biol. 2022;2427:167-175. doi: 10.1007/978-1-0716-1971-1_14.
Human infections by gastrointestinal bacterial pathogens are commonly associated with the consumption of contaminated food of animal origin (e.g., chicken, fish, eggs) or contaminated water. However, further contamination sources must be considered since number of Salmonella enterica infections associated with the consumption of food of non-animal origin (e.g., vegetables, fruits, nuts) are increasing. This gives raise to interest in understanding the interaction of S. enterica with leafy produce, such as various salads. Especially adhesion as initial step of contamination of salad by S. enterica deserves further investigation. Here we introduce methods to analyse Salmonella adhesion to various salads that provide insights into bacterial factors involved in Salmonella colonization of plants.
人类胃肠道细菌病原体感染通常与食用受污染的动物源性食物（如鸡肉、鱼类、蛋类）或污染的水有关。然而，由于与食用非动物源性食物（如蔬菜、水果、坚果）相关的沙门氏菌感染数量不断增加，因此必须考虑其他污染来源。这引起了人们对了解沙门氏菌与叶类蔬菜（如各种沙拉）相互作用的兴趣。特别是作为沙门氏菌污染沙拉的初始步骤的黏附作用更值得进一步研究。在这里，我们介绍了分析沙门氏菌黏附到各种沙拉的方法，这些方法为了解沙门氏菌在植物定殖中涉及的细菌因素提供了线索。
