Chitnis M P, Kamath N S
Oncology. 1987;44(1):47-50. doi: 10.1159/000226442.
The effect of dipyrone along with doxorubicin and mitoxantrone was studied alone and in combination on the 3H-thymidine (3H-TdR) incorporation in P388 leukaemia sensitive (P388/S) and resistant (P388/ADR) to doxorubicin. Dipyrone 10(-4) M demonstrated minimal inhibitory effect on DNA biosynthesis in both the sensitive and resistant cells. Doxorubicin and mitoxantrone at equimolar concentrations, indicated time and dose-dependent inhibition in 3H-TdR incorporation in the sensitive cells. The inhibition was more at the higher drug concentrations at 4 h drug exposure. Mitoxantrone showed cross-resistance in P388/ADR compared to P388/S. Both the drugs along with 10(-4) M dipyrone in the incubating medium revealed synergistic inhibitory activity in P388/S and P388/ADR. Observations indicate circumvention of doxorubicin and mitoxantrone resistance in P388/ADR by dipyrone.
研究了安乃近与阿霉素和米托蒽醌单独及联合使用时，对阿霉素敏感（P388/S）和耐药（P388/ADR）的P388白血病细胞掺入3H-胸腺嘧啶核苷（3H-TdR）的影响。10(-4) M的安乃近对敏感和耐药细胞的DNA生物合成均显示出最小抑制作用。等摩尔浓度的阿霉素和米托蒽醌对敏感细胞中3H-TdR的掺入表现出时间和剂量依赖性抑制。在药物暴露4小时时，较高药物浓度下的抑制作用更强。与P388/S相比，米托蒽醌在P388/ADR中表现出交叉耐药性。孵育培养基中这两种药物与10(-4) M安乃近一起，在P388/S和P388/ADR中均显示出协同抑制活性。观察结果表明安乃近可克服P388/ADR对阿霉素和米托蒽醌的耐药性。
