Li Xin, Wang Li, Ma Shaojun, Lin Shaohui, Wang Chunyan, Wang Haiya
Department of General Medicine and Geriatrics, The Second Affiliated Hospital of School of Medicine, Linping Campus, Zhejiang University, No. 369, Yingbin Rd, Nanyuan St, Linping District, Hangzhou, Zhejiang Province 311100, China.
Department of Geriatrics, Renji Hospital, School of Medicine, Shanghai Jiaotong University, No. 145, Shandong Rd., Shanghai 200001, China.
Int J Hypertens. 2022 May 17;2022:5999530. doi: 10.1155/2022/5999530. eCollection 2022.
The human microbiome is a mixed group of microorganisms, which individually consists of 10-100 trillion symbiotic microbial cells. The relationship between gastrointestinal microbiota and blood pressure has been verified and the intestinal microbiota of chronic kidney disease (CKD) patients in the distribution of bacterial species is different from the flora of people with no CKD. The purpose of this research is to study the different intestinal microbiota of hypertensive patients with and without nephropathy and to find possible biomarkers of hypertensive nephropathy (H-CKD). The subjects of this research were divided into three groups, healthy control group, hypertension group, and hypertensive nephropathy group. Sequencing, bioinformatics, and statistical analysis were performed on the 16S rRNA gene of the subjects' stool samples. This research study showed the differences of intestinal flora as biomarkers in hypertension patients with and without nephropathy; it investigated the relationship of the differences in the intestinal microbiota with bile-acid metabolism; it also explored bile-acid metabolism mechanism of intestinal microbiota differences in hypertension with or without nephropathy. In summary, the difference in the combination of and in the gastrointestinal microbiota is related to bile-acid metabolism in hypertensive patients and can be one of the factors causing CKD. It is the first time to report such a biomarker or pathogenic factor of H-CKD in the world.
人类微生物群是一组混合的微生物，其个体由10万亿至100万亿个共生微生物细胞组成。胃肠道微生物群与血压之间的关系已得到证实，慢性肾脏病（CKD）患者的肠道微生物群在细菌种类分布上与无CKD的人群不同。本研究的目的是研究有肾病和无肾病的高血压患者肠道微生物群的差异，并寻找高血压肾病（H-CKD）可能的生物标志物。本研究的受试者分为三组，即健康对照组、高血压组和高血压肾病组。对受试者粪便样本的16S rRNA基因进行测序、生物信息学分析和统计分析。本研究表明，有肾病和无肾病的高血压患者肠道菌群作为生物标志物存在差异；研究了肠道微生物群差异与胆汁酸代谢的关系；还探讨了有肾病和无肾病高血压患者肠道微生物群差异的胆汁酸代谢机制。总之，胃肠道微生物群中[具体物质1]和[具体物质2]组合的差异与高血压患者的胆汁酸代谢有关，可能是导致CKD的因素之一。这是首次在世界范围内报道此类H-CKD的生物标志物或致病因素。
