An approach to DNA fragment synthesis from unprotected nucleoside.
作者信息
Shimidzu T, Ozaki H, Yamoto S, Maikuma S, Honda K
出版信息
Nucleic Acids Symp Ser. 1986(17):81-4.
PMID:3562282
Abstract
Oligodeoxynucleotides have been synthesized from unprotected nucleoside by use of morpholinophosphordichloridite as a phosphorylating reagent. This procedure consists of three (in situ) steps; the reaction of 5'-O-protected deoxynucleotide with morpholinophosphordichloridite, the reaction of the resulting on active mononucleotide derivative with the second nucleoside, non-aqueous oxidation, and then removal of amino moiety at phosphate diester linkage.
摘要
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