Sicilia Angelo, Russo Riccardo, Caruso Marco, Arlotta Carmen, Di Silvestro Silvia, Gmitter Frederick G, Gentile Alessandra, Nicolosi Elisabetta, Lo Piero Angela Roberta
Department of Agriculture, Food and Environment, University of Catania, Via Santa Sofia 98, 95123 Catania, Italy.
Citrus Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Lake Alfred, FL 33809, USA.
Biology (Basel). 2022 May 17;11(5):761. doi: 10.3390/biology11050761.
The causal agent of mal secco disease is the fungus , mainly affecting lemon tree survival in the Mediterranean area. Using a fully compatible host-pathogen interaction, the aim of our work was to retrieve the fungus transcriptome by an RNA seq approach during infection of rough lemon ( Lush.) to identify crucial transcripts for pathogenesis establishment and progression. A total of 2438 clusters belonging to were retrieved and classified into the GO and KEGG categories. Transcripts were categorized mainly within the "membrane", "catalytic activity", and "primary metabolic process" GO terms. Moreover, most of the transcripts are included in the "ribosome", "carbon metabolism", and "oxidative phosphorylation" KEGG categories. By focusing our attention on transcripts with FPKM values higher than the median, we were able to identify four main transcript groups functioning in (a) fungus cell wall remodeling and protection, (b) destroying plant defensive secondary metabolites, (c) optimizing fungus development and pathogenesis, and (d) toxin biosynthesis, thus indicating that a multifaceted strategy to subdue the host was executed.
黑腐病的致病因子是一种真菌，主要影响地中海地区柠檬树的存活。利用完全兼容的宿主 - 病原体相互作用，我们研究的目的是通过RNA测序方法在粗柠檬（Lush.）感染期间获取真菌转录组，以鉴定发病机制建立和进展的关键转录本。总共检索到属于 的2438个聚类，并将其分类到GO和KEGG类别中。转录本主要归类于“膜”、“催化活性”和“初级代谢过程”的GO术语中。此外，大多数转录本包含在“核糖体”、“碳代谢”和“氧化磷酸化”的KEGG类别中。通过关注FPKM值高于中位数的转录本，我们能够识别出四个主要转录本组，它们分别在（a）真菌细胞壁重塑和保护、（b）破坏植物防御性次生代谢产物、（c）优化真菌发育和发病机制以及（d）毒素生物合成中发挥作用，从而表明执行了一种多方面制服宿主的策略。
