Vascularized Composite Allotransplantation (VCA) Laboratory, Department of Plastic and Reconstructive Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Department of Hand and Microsurgery, Xiangya Hospital, Central South University, Changsha 410017, China.
Cells. 2022 May 16;11(10):1653. doi: 10.3390/cells11101653.
Static cold storage is the cheapest and easiest method and current gold standard to store and preserve donor organs. This study aimed to compare the preservative capacity of gluconate-lactobionate-dextran (Unisol) solutions to histidine-tryptophan-ketoglutarate (HTK) solution. Murine syngeneic heterotopic heart transplantations (Balb/c-Balb/c) were carried out after 18 h of static cold storage. Cardiac grafts were either flushed and stored with Unisol-based solutions with high-(UHK) and low-potassium (ULK) ± glutathione, or HTK. Cardiac grafts were assessed for rebeating and functionality, histomorphologic alterations, and cytokine expression. Unisol-based solutions demonstrated a faster rebeating time (UHK 56 s, UHK + Glut 44 s, ULK 45 s, ULK + Glut 47 s) compared to HTK (119.5 s) along with a better contractility early after reperfusion and at the endpoint on POD 3. Ischemic injury led to a significantly increased leukocyte recruitment, with similar degrees of tissue damage and inflammatory infiltrate in all groups, yet the number of apoptotic cells tended to be lower in ULK compared to HTK. In UHK- and ULK-treated animals, a trend toward decreased expression of proinflammatory markers was seen when compared to HTK. Unisol-based solutions showed an improved preservative capacity compared with the gold standard HTK early after cardiac transplantation. Supplemented glutathione did not further improve tissue-protective properties.
静态冷保存是最便宜、最简单的方法，也是目前保存和保存供体器官的黄金标准。本研究旨在比较葡萄糖酸盐-乳糖酸盐-葡聚糖（Unisol）溶液和组氨酸-色氨酸-酮戊二酸（HTK）溶液的保存能力。在静态冷保存 18 小时后，进行鼠同源异基因异位心脏移植。心脏移植物用基于 Unisol 的高钾（UHK）和低钾（ULK）±谷胱甘肽溶液或 HTK 冲洗和保存。评估心脏移植物的再跳动和功能、组织形态改变和细胞因子表达。与 HTK（119.5 s）相比，基于 Unisol 的溶液具有更快的再跳动时间（UHK 56 s，UHK + Glut 44 s，ULK 45 s，ULK + Glut 47 s），并且在再灌注早期和 POD 3 终点时具有更好的收缩性。缺血损伤导致白细胞募集显著增加，所有组的组织损伤和炎症浸润程度相似，但与 HTK 相比，ULK 中的细胞凋亡数量较低。与 HTK 相比，在 UHK 和 ULK 处理的动物中，促炎标志物的表达呈下降趋势。与金标准 HTK 相比，基于 Unisol 的溶液在心脏移植后早期显示出更好的保存能力。补充谷胱甘肽并没有进一步改善组织保护特性。
