College of Horticulture, Anhui Agricultural University, Hefei 230036, China.
Genes (Basel). 2022 May 11;13(5):855. doi: 10.3390/genes13050855.
The WRKY transcription factors (TFs) participate in various physiological, growth and developmental processes of plants. In our study, a total of 79 WRKY family members were identified and classified into three groups (Group I, Group IIa-e, and Group III) based on phylogenetic and conservative domain analyses. Conserved motif analysis showed that seven WRKYGQK domains changed. The promoter sequence analysis suggested that there were multiple stress- and hormone-related cis-regulatory elements in the promoter regions of genes. Expression patterns of based on RNA-seq data revealed their diverse expression profiles in five tissues and under different treatments. Subcellular localization analysis showed that PhWRKY71 was located in the nucleus. In addition, overexpression of caused a significant increase in branch number. This indicated that played a critical role in regulating the shoot branching of . The above results lay the foundation for further revealing the functions of genes.
WRKY 转录因子(TFs)参与植物的各种生理、生长和发育过程。在我们的研究中,根据系统发生和保守结构域分析,共鉴定出 79 个 WRKY 家族成员,并将其分为三组(I 组、IIa-e 组和 III 组)。保守基序分析表明,有七个 WRKYGQK 结构域发生了变化。启动子序列分析表明,基因启动子区域存在多种与应激和激素相关的顺式调控元件。基于 RNA-seq 数据的表达模式显示,它们在五种组织和不同处理下具有不同的表达谱。亚细胞定位分析表明 PhWRKY71 位于细胞核中。此外,过表达 导致分支数显著增加。这表明 在调节 的芽分枝中起关键作用。上述结果为进一步揭示 基因的功能奠定了基础。