Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Ji'nan 250014, China.
Int J Mol Sci. 2022 May 12;23(10):5401. doi: 10.3390/ijms23105401.
With global increases in saline soil, it has become increasingly important to decipher salt-tolerance mechanisms and identify strategies to improve salt tolerance in crops. Halophytes complete their life cycles in environments containing ≥200 mM NaCl; these remarkable plants provide a potential source of genes for improving crop salt tolerance. Recretohalophytes such as have salt glands that secrete Na on their leaf epidermis. Here, we identified , an uncharacterized gene with no conserved domains, from , which was highly expressed after NaCl treatment. We confirmed its expression in the salt gland by in situ hybridization, and then heterologously expressed in . The transgenic lines had a higher germination rate, greater cotyledon growth percentage, and longer roots than the wild type (WT) under NaCl treatments (50, 100 and 150 mM). At the seedling stage, the transgenic lines grew better than the WT and had lower Na and malonyldialdehyde accumulation, and higher K and proline contents. This corresponded with the high expression of the key proline biosynthesis genes and under NaCl treatment. Isotonic mannitol treatment showed that overexpression significantly relieved osmotic stress. Therefore, this novel gene provides a potential target for improving salt tolerance.
随着全球盐渍土的增加，破译耐盐机制和确定提高作物耐盐性的策略变得越来越重要。盐生植物在含有≥200mM NaCl 的环境中完成其生命周期；这些非凡的植物为提高作物耐盐性提供了潜在的基因来源。再盐生植物如 具有在其叶片表皮上分泌 Na 的盐腺。在这里，我们从 中鉴定出一个未被表征的基因，它没有保守结构域，在 NaCl 处理后高度表达。我们通过原位杂交证实了其在盐腺中的表达，然后在 中异源表达 。与野生型（WT）相比，在 NaCl 处理（50、100 和 150mM）下，转基因系具有更高的萌发率、更大的子叶生长百分比和更长的根。在幼苗阶段，转基因系比 WT 生长得更好，Na 和丙二醛积累较低，K 和脯氨酸含量较高。这与 NaCl 处理下关键脯氨酸生物合成基因 和 的高表达相对应。等渗甘露醇处理表明，过表达 显著缓解了渗透胁迫。因此，这个新基因提供了一个提高耐盐性的潜在目标。
