Yu Jingya, Zhang Haibin, Zhang Yan, Zhang Xiaolu
Department of Diagnostics, Medical Integration and Practice Center, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
Life (Basel). 2022 May 7;12(5):701. doi: 10.3390/life12050701.
Hepatitis B virus (HBV) infection is the most prominent risk factor for developing hepatocellular carcinoma (HCC), which can increase the incidence of HCC by more than 100 times. Accumulated evidence has revealed that non-coding RNAs (ncRNAs) play a regulatory role in various tumors through the long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA regulation axis. However, the involvement of the ncRNA regulatory network in the progression of HBV infection-induced HCC remains elusive. In the current work, five tumor samples from patients with hepatitis B surface antigen (HBsAg)-positive HCC and three tumor samples from patients with HBsAg-negative HCC were collected for whole-transcriptome sequencing. Between the two groups, 841 lncRNAs, 54 miRNAs, and 1118 mRNAs were identified to be differentially expressed (DE). The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that DE genes were mainly involved in cancer-related pathways, including Wnt and MAPK signaling pathways. The Gene Expression Omnibus (GEO) analysis further validated the selected DE mRNAs. The DE lncRNA-miRNA-mRNA network was built to explore the effect of HBV infection on the regulation of ncRNAs in HCC. These findings provide novel insights into the role of HBV infection in the progression of HCC.
乙型肝炎病毒（HBV）感染是发生肝细胞癌（HCC）最主要的危险因素，可使HCC的发病率增加100多倍。越来越多的证据表明，非编码RNA（ncRNA）通过长链非编码RNA（lncRNA）-微小RNA（miRNA）-信使核糖核酸（mRNA）调控轴在各种肿瘤中发挥调节作用。然而，ncRNA调控网络在HBV感染诱导的HCC进展中的作用仍不清楚。在当前研究中，收集了5例乙型肝炎表面抗原（HBsAg）阳性HCC患者的肿瘤样本和3例HBsAg阴性HCC患者的肿瘤样本进行全转录组测序。在两组之间，共鉴定出841种lncRNA、54种miRNA和1118种mRNA存在差异表达（DE）。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，差异表达基因主要参与癌症相关途径，包括Wnt和丝裂原活化蛋白激酶（MAPK）信号通路。基因表达综合数据库（GEO）分析进一步验证了所选的差异表达mRNA。构建差异表达lncRNA-miRNA-mRNA网络以探讨HBV感染对HCC中ncRNA调控的影响。这些发现为HBV感染在HCC进展中的作用提供了新的见解。
