Lin Shu-Hsien, Wang Chih-Chi, Huang Kuang-Tzu, Chen Kuang-Den, Hsu Li-Wen, Eng Hock-Liew, Chiu King-Wah
Division of Hepato-Gastroenterology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan.
Liver Transplantation Program, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung 83301, Taiwan.
Metabolites. 2022 Apr 25;12(5):388. doi: 10.3390/metabo12050388.
Most cases of advanced liver diseases are associated with low serum 25-hydroxyvitamin D and vitamin D deficiency. This phenomenon may occur in living donor liver transplantation (LDLT).
We conducted this study to explore the interplay between VDR and CYP2R1 in liver graft and compared our findings with the pathological interpretation of serum 25(OH)D concentration.
In total, 60 patients received liver graft biopsy after LDLT and were separated (1:1) into two groups: graft rejection group and graft non-rejection group. We extracted both of the recipients' and donors' serum DNA to investigate the vitamin D receptor (VDR) rs2228530 and CYP2R1 rs10741657 single nucleotide polymorphisms (SNPs) using real-time polymerase chain reaction. We also extracted DNA from liver graft tissues to explore the genetic alleles of VDR rs2228530 and CYP2R1 rs10741657 after LDLT. Serum biochemistry profile and 25(OH)D concentrations were measured before and after LDLT.
There were no significant differences in serum VDR rs2228530 and CYP2R1 rs10741657 genetic alleles between recipients and donors. The percentage of genetic modification was 33.4% (10/30) for the rejection and non-rejection groups in VDR rs2228530, and 66.7% (20/30) for both groups in CYP2R1 rs10741657. Serum 25(OH)D concentrations were significantly lower after LDLT D30 than that before LDLT in the rejection ( = 0.0001) and non-rejection graft pathology ( = 0.0017) groups.
The presence of low serum 25(OH)D concentrations after LDLT suggested that post-transplant low serum 25(OH)D concentrations may develop with the homogenous phenomenon of VDR rs2228530 and CYP2R1 rs10741657 genetic modifications in recipients regardless of graft pathology.
大多数晚期肝病病例与血清25-羟基维生素D水平低和维生素D缺乏有关。这种现象可能发生在活体肝移植（LDLT）中。
我们开展本研究以探讨肝移植中维生素D受体（VDR）和细胞色素P450 2R1（CYP2R1）之间的相互作用，并将我们的研究结果与血清25（OH）D浓度的病理学解释进行比较。
共有60例患者在LDLT后接受了肝移植活检，并被（1:1）分为两组：移植排斥组和移植非排斥组。我们提取了受者和供者的血清DNA，使用实时聚合酶链反应研究维生素D受体（VDR）rs2228530和CYP2R1 rs10741657单核苷酸多态性（SNP）。我们还从肝移植组织中提取DNA，以探究LDLT后VDR rs2228530和CYP2R1 rs10741657的基因等位基因。在LDLT前后测量血清生化指标和25（OH）D浓度。
受者和供者之间血清VDR rs2228530和CYP2R1 rs10741657基因等位基因无显著差异。在VDR rs2228530中，排斥组和非排斥组的基因修饰百分比分别为33.4%（10/30），在CYP2R1 rs10741657中，两组均为66.7%（20/30）。在移植排斥（P = 0.0001）和移植非排斥病理（P = 0.0017）组中，LDLT后30天的血清25（OH）D浓度显著低于LDLT前。
LDLT后血清25（OH）D浓度低表明，无论移植病理如何，移植后血清25（OH）D浓度低可能与受者中VDR rs2228530和CYP2R1 rs10741657基因修饰的同质现象有关。
