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使用CXCR4靶向磁性荧光纳米探针单芯片分离耐药急性髓系白血病细胞

One-Chip Isolation of Drug-Resistant Acute Myeloid Leukemia Cells with CXCR4-Targeted Magnetic Fluorescent Nanoprobes.

作者信息

Wang Fan, Jiang Yuqi, Wang Luhai, Chen Yi, Zhang Yu, Ma Ming

机构信息

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210096, China.

出版信息

Nanomaterials (Basel). 2022 May 17;12(10):1711. doi: 10.3390/nano12101711.

DOI:10.3390/nano12101711
PMID:35630929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9142899/
Abstract

Drug resistance and relapse lead to high mortality in acute myeloid leukemia, and studies have shown that CXCR4 overexpression is highly correlated with poor prognosis and drug resistance in leukemia cells. Isolation and detection of AML cells with CXCR4 overexpression will be crucial to the treatment of AML. In this paper, magnetic nanoparticles were firstly prepared successfully by high-temperature thermal decomposition method, and then characterized by TEM, VSM and DLS. Subsequently CXCR4-targeted magnetic fluorescent nanoprobes conjugated with antibody 12G5 were constructed by stepwise coupling. In cell experiments, the obtained probes demonstrated excellent targeting efficacy to CXCR4 overexpressed AML cells HL-60. In addition, HL-60 cells labelled with the magnetic probes can be magnetic isolated successfully in one microfluidics chip, with efficiency of 82.92 ± 7.03%. Overall, this method utilizes the superiority of superparamagnetic nanomaterials and microfluidic technology to achieve the enrichment and capture of drug-resistant cells in a microfluidic chip, providing a new idea for the isolation and detective of drug-resistant acute myeloid leukemia cells.

摘要

耐药性和复发导致急性髓系白血病的高死亡率，研究表明CXCR4过表达与白血病细胞的不良预后和耐药性高度相关。分离和检测CXCR4过表达的急性髓系白血病细胞对急性髓系白血病的治疗至关重要。本文首先通过高温热分解法成功制备了磁性纳米颗粒，然后通过透射电子显微镜、振动样品磁强计和动态光散射对其进行了表征。随后，通过逐步偶联构建了与抗体12G5偶联的CXCR4靶向磁性荧光纳米探针。在细胞实验中，所获得的探针显示出对CXCR4过表达的急性髓系白血病细胞HL-60具有优异的靶向效果。此外，用磁性探针标记的HL-60细胞能够在一个微流控芯片中成功地进行磁性分离，分离效率为82.92±7.03%。总体而言，该方法利用超顺磁性纳米材料和微流控技术的优势，在微流控芯片中实现耐药细胞的富集和捕获，为耐药急性髓系白血病细胞的分离和检测提供了新思路。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/8d836ca5000e/nanomaterials-12-01711-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/f32999926f57/nanomaterials-12-01711-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/7f3e290a41ae/nanomaterials-12-01711-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/30d17c5113d2/nanomaterials-12-01711-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/df014f985994/nanomaterials-12-01711-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/2862452175ce/nanomaterials-12-01711-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/4111eeb1f574/nanomaterials-12-01711-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/9a071c05b44e/nanomaterials-12-01711-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/5ca0b7797efb/nanomaterials-12-01711-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/8d836ca5000e/nanomaterials-12-01711-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/f32999926f57/nanomaterials-12-01711-sch001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/7f3e290a41ae/nanomaterials-12-01711-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/30d17c5113d2/nanomaterials-12-01711-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/df014f985994/nanomaterials-12-01711-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/2862452175ce/nanomaterials-12-01711-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/4111eeb1f574/nanomaterials-12-01711-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/9a071c05b44e/nanomaterials-12-01711-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/5ca0b7797efb/nanomaterials-12-01711-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6181/9142899/8d836ca5000e/nanomaterials-12-01711-g008.jpg

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