Zhang Cangheng, Li Yan, Xing Shu, Yang Xiaodeng, Zhao Jinrong, Dong Qiaoyan
Shandong Provincial Key Laboratory of Fine Chemicals, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
Rocket Force Sergeancy School, Weifang 260000, China.
Polymers (Basel). 2022 May 10;14(10):1936. doi: 10.3390/polym14101936.
Chitosan has potential applications in many fields, due to its biocompatibility, biodegradability and reproducibility. However, the insolubility in water restricts its wide application. In order to expand the application of chitosan in the delivery of oil-soluble drugs and improve the efficacy of oil-soluble drugs, -Glycidyltrimethyl ammonium chloride-modified chitosan (GTA-m-CS) and ,-Dimethyl--dodecyl--(1,2-epoxy propyl) ammonium chloride (DDEAC), a kind of reactive surfactant, were synthesized and characterized by FTIR, NMR and XRD methods. The interactions between GTA-m-CS and DDEAC was studied by surface tension, viscosity, conductivity and fluorescence methods. The parameters, including equilibrium surface tension, critical micelle concentrations of DDEAC with different GTA-m-CS concentration, critical aggregation concentration of DDEAC, the amount of DDEAC adsorbed on GTA-m-CS, c and were obtained from the surface tension curves. The influence of temperature on the above parameters were evaluated. The degree of counterion binding to micelle and the thermodynamic parameters of the system were calculated from the conductivity curves. According to the change of conductivity with temperature, the thermodynamic parameters of micellar formation were calculated. The aggregation number of DDEAC molecules in GTA-m-CS/DDEAC aggregates were calculated from steady-state fluorescence data. Based on the experimental results, the interaction models between GTA-m-CS and DDEAC were proposed. The GTA-m-CS/DDEAC aggregates could be used as curcumin carries, and achieved sustained release.
壳聚糖因其生物相容性、生物降解性和可再生性在许多领域具有潜在应用。然而，其在水中的不溶性限制了其广泛应用。为了扩大壳聚糖在油溶性药物递送中的应用并提高油溶性药物的疗效，合成了缩水甘油三甲基氯化铵改性壳聚糖（GTA-m-CS）和一种反应性表面活性剂二甲基十二烷基（1,2-环氧丙基）氯化铵（DDEAC），并通过傅里叶变换红外光谱（FTIR）、核磁共振（NMR）和X射线衍射（XRD）方法对其进行了表征。通过表面张力、粘度、电导率和荧光方法研究了GTA-m-CS与DDEAC之间的相互作用。从表面张力曲线获得了包括平衡表面张力、不同GTA-m-CS浓度下DDEAC的临界胶束浓度、DDEAC的临界聚集浓度、吸附在GTA-m-CS上的DDEAC的量、c和等参数。评估了温度对上述参数的影响。从电导率曲线计算了反离子与胶束的结合程度和体系的热力学参数。根据电导率随温度的变化，计算了胶束形成的热力学参数。从稳态荧光数据计算了GTA-m-CS/DDEAC聚集体中DDEAC分子的聚集数。基于实验结果，提出了GTA-m-CS与DDEAC之间的相互作用模型。GTA-m-CS/DDEAC聚集体可作为姜黄素载体，并实现缓释。
